The sonication-assisted whisker method enables CRISPR-Cas9 ribonucleoprotein delivery to induce genome editing in rice

The sonication-assisted whisker method enables CRISPR-Cas9 ribonucleoprotein delivery to induce genome editing in rice

Abstract CRISPR/Cas9-based genome editing represents an unprecedented potential for plant breeding. Unlike animal cells, plant cells contain a rigid cell wall, genome editing tool delivery into plant cells is thus challenging. In particular, the delivery of the Cas9-gRNA ribonucleoprotein (RNP) into...

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Bibliographic Details
Main Authors: Akiyoshi Nakamura, Tsubasa Yano, Nobutaka Mitsuda, Maiko Furubayashi, Seiichiro Ito, Shigeo S. Sugano, Teruhiko Terakawa
Format: Article
Language:English
Published: Nature Portfolio 2023-09-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-023-40433-w

Internet

https://doi.org/10.1038/s41598-023-40433-w

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