Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats
This study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6) and control (n = 6). After splenectomy (15 days), the administration of 0.1ml of 99mTc-DMSA IV (0.48 MBq) was carr...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2008-12-01
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| Series: | Brazilian Archives of Biology and Technology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700032 |
| _version_ | 1818562766593589248 |
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| author | Maria Kadja Meneses Torres Açucena Kércia Regina Santos Gomes Pereira Arthur Villarim Neto Amália Cínthia Meneses Rêgo Mario Bernardo-Filho Ítalo Medeiros Azevedo Irami Araújo Filho Aldo Cunha Medeiros |
| author_facet | Maria Kadja Meneses Torres Açucena Kércia Regina Santos Gomes Pereira Arthur Villarim Neto Amália Cínthia Meneses Rêgo Mario Bernardo-Filho Ítalo Medeiros Azevedo Irami Araújo Filho Aldo Cunha Medeiros |
| author_sort | Maria Kadja Meneses Torres Açucena |
| collection | DOAJ |
| description | This study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6) and control (n = 6). After splenectomy (15 days), the administration of 0.1ml of 99mTc-DMSA IV (0.48 MBq) was carried out. Thirty minutes later, kidney, heart, lung, thyroid, stomach, bladder and femur and samples of blood were isolated. The organs were weighed, counted and the percentage of radioactivity /g (%ATI/g) determined. Serum urea and creatinine, hematocrit, leukocytes and platelets were measured. Statistics by t test (p<0.05) was done. There was a significant reduction in %ATI/g in kidney and blood (p<0.05) of splenectomized animals, a significant increase (p<0.05) of urea (88.8 ± 18.6 mg/dL) and creatinine (0.56 ± 0.08 mg/dL), compared to the controls (51.5±1.6, 0.37±0.02mg/dL, respectively), as well as increase in platelets and leucocytes, and hematocrit reduction. The analysis of the results indicates that in rats, splenectomy seems to alter the renal function and the uptake of 99mTc-DMSA.<br>Estudo com objetivo de avaliar se a esplenectomia altera a biodistribuição do 99mTc-DMSA e alguns parâmetros bioquímicos e hematológicos em ratos Wistar. Os animais forma divididos em 2 grupos: esplenectomizados (n=6) e controle(n=6). Após 15 dias, administração de 0,1 ml de 99mTc-DMSA via plexo orbital (0,48 MBq) foi realizada. Rim, coração, pulmão, tireóide, estômago, bexiga e fêmur e amostras de sangue foram separadas. Após pesagem e contagem da radioatividade foi determinado o percentual de radioatividade/g (% ATI/g). Dosadas uréia e creatinina sérica, hematócrito, plaquetas e leucócitos. Estatística pelo teste t, significância 0,05 foi realizada. Foi observada redução significante no %ATI/g no rim e sangue (p<0,05) dos animais esplenectomizados, aumento significante (p<0.05) da uréia (88,8±18,6 mg/dL) e creatinina (0,56±0,08), comparado aos controles (51,5±1,6; 0,37±0,02mg/dL, respectivamente) assim como aumento de leucócitos e plaquetas e redução de hematócrito. Conclui-se que em ratos, a esplenectomia alterou a captação de 99mTc-DMSA pelo rim, e a função renal. |
| first_indexed | 2024-12-14T01:08:04Z |
| format | Article |
| id | doaj.art-44496cf1215f462dbc991a5bef063783 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-14T01:08:04Z |
| publishDate | 2008-12-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-44496cf1215f462dbc991a5bef0637832022-12-21T23:22:52ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242008-12-0151spe19720210.1590/S1516-89132008000700032Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in ratsMaria Kadja Meneses Torres AçucenaKércia Regina Santos Gomes PereiraArthur Villarim NetoAmália Cínthia Meneses RêgoMario Bernardo-FilhoÍtalo Medeiros AzevedoIrami Araújo FilhoAldo Cunha MedeirosThis study aimed to evaluate if the splenectomy alters the biodistribution of 99mTc-DMSA and renal function in Wistar rats. The animals were separated in the groups: splenectomy (n = 6) and control (n = 6). After splenectomy (15 days), the administration of 0.1ml of 99mTc-DMSA IV (0.48 MBq) was carried out. Thirty minutes later, kidney, heart, lung, thyroid, stomach, bladder and femur and samples of blood were isolated. The organs were weighed, counted and the percentage of radioactivity /g (%ATI/g) determined. Serum urea and creatinine, hematocrit, leukocytes and platelets were measured. Statistics by t test (p<0.05) was done. There was a significant reduction in %ATI/g in kidney and blood (p<0.05) of splenectomized animals, a significant increase (p<0.05) of urea (88.8 ± 18.6 mg/dL) and creatinine (0.56 ± 0.08 mg/dL), compared to the controls (51.5±1.6, 0.37±0.02mg/dL, respectively), as well as increase in platelets and leucocytes, and hematocrit reduction. The analysis of the results indicates that in rats, splenectomy seems to alter the renal function and the uptake of 99mTc-DMSA.<br>Estudo com objetivo de avaliar se a esplenectomia altera a biodistribuição do 99mTc-DMSA e alguns parâmetros bioquímicos e hematológicos em ratos Wistar. Os animais forma divididos em 2 grupos: esplenectomizados (n=6) e controle(n=6). Após 15 dias, administração de 0,1 ml de 99mTc-DMSA via plexo orbital (0,48 MBq) foi realizada. Rim, coração, pulmão, tireóide, estômago, bexiga e fêmur e amostras de sangue foram separadas. Após pesagem e contagem da radioatividade foi determinado o percentual de radioatividade/g (% ATI/g). Dosadas uréia e creatinina sérica, hematócrito, plaquetas e leucócitos. Estatística pelo teste t, significância 0,05 foi realizada. Foi observada redução significante no %ATI/g no rim e sangue (p<0,05) dos animais esplenectomizados, aumento significante (p<0.05) da uréia (88,8±18,6 mg/dL) e creatinina (0,56±0,08), comparado aos controles (51,5±1,6; 0,37±0,02mg/dL, respectivamente) assim como aumento de leucócitos e plaquetas e redução de hematócrito. Conclui-se que em ratos, a esplenectomia alterou a captação de 99mTc-DMSA pelo rim, e a função renal.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700032SplenectomyBiodistributionTechnetiumRats |
| spellingShingle | Maria Kadja Meneses Torres Açucena Kércia Regina Santos Gomes Pereira Arthur Villarim Neto Amália Cínthia Meneses Rêgo Mario Bernardo-Filho Ítalo Medeiros Azevedo Irami Araújo Filho Aldo Cunha Medeiros Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats Brazilian Archives of Biology and Technology Splenectomy Biodistribution Technetium Rats |
| title | Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats |
| title_full | Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats |
| title_fullStr | Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats |
| title_full_unstemmed | Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats |
| title_short | Influence of splenectomy on the biodistribution of technetium-99m dimercaptosuccinic acid (99mTc-DMSA) in rats |
| title_sort | influence of splenectomy on the biodistribution of technetium 99m dimercaptosuccinic acid 99mtc dmsa in rats |
| topic | Splenectomy Biodistribution Technetium Rats |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700032 |
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