Opportunities and short-comings of the axolotl salamander heart as a model system of human single ventricle and excessive trabeculation
Abstract Few experimental model systems are available for the rare congenital heart diseases of double inlet left ventricle (DILV), a subgroup of univentricular hearts, and excessive trabeculation (ET), or noncompaction. Here, we explore the heart of the axolotl salamander (Ambystoma mexicanum, Shaw...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-24442-9 |
| _version_ | 1797985536927858688 |
|---|---|
| author | Sophie Meyer Henrik Lauridsen Kathrine Pedersen Sofie Amalie Andersson Pim van Ooij Tineke Willems Rolf M. F. Berger Tjark Ebels Bjarke Jensen |
| author_facet | Sophie Meyer Henrik Lauridsen Kathrine Pedersen Sofie Amalie Andersson Pim van Ooij Tineke Willems Rolf M. F. Berger Tjark Ebels Bjarke Jensen |
| author_sort | Sophie Meyer |
| collection | DOAJ |
| description | Abstract Few experimental model systems are available for the rare congenital heart diseases of double inlet left ventricle (DILV), a subgroup of univentricular hearts, and excessive trabeculation (ET), or noncompaction. Here, we explore the heart of the axolotl salamander (Ambystoma mexicanum, Shaw 1789) as model system of these diseases. Using micro-echocardiography, we assessed the form and function of the heart of the axolotl, an amphibian, and compared this to human DILV (n = 3). The main finding was that both in the axolotl and DILV, blood flows of disparate oxygen saturation can stay separated in a single ventricle. In the axolotl there is a solitary ventricular inlet and outlet, whereas in DILV there are two separate inlets and outlets. Axolotls had a lower resting heart rate compared to DILV (22 vs. 72 beats per minute), lower ejection fraction (47 vs. 58%), and their oxygen consumption at rest was higher than peak oxygen consumption in DILV (30 vs. 17 ml min−1 kg−1). Concerning the ventricular myocardial organization, histology showed trabeculations in ET (n = 5) are much closer to the normal human setting than to the axolotl setting. We conclude that the axolotl heart resembles some aspects of DILV and ET albeit substantial species differences exist. |
| first_indexed | 2024-04-11T07:18:36Z |
| format | Article |
| id | doaj.art-4462126a45294135b23845e9d0ad1bdb |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T07:18:36Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-4462126a45294135b23845e9d0ad1bdb2022-12-22T04:37:49ZengNature PortfolioScientific Reports2045-23222022-11-0112111310.1038/s41598-022-24442-9Opportunities and short-comings of the axolotl salamander heart as a model system of human single ventricle and excessive trabeculationSophie Meyer0Henrik Lauridsen1Kathrine Pedersen2Sofie Amalie Andersson3Pim van Ooij4Tineke Willems5Rolf M. F. Berger6Tjark Ebels7Bjarke Jensen8Center for Congenital Heart Diseases, Department of Cardiothoracic Surgery, University Medical Center GroningenDepartment of Clinical Medicine, Aarhus UniversityDepartment of Clinical Medicine, Aarhus UniversityDepartment of Clinical Medicine, Aarhus UniversityDepartment of Radiology and Nuclear Medicine, Amsterdam University Medical Centers, location AMCDepartment of Radiology, University Medical Center GroningenCenter for Congenital Heart Diseases, Department of Pediatric Cardiology, University Medical Center GroningenCenter for Congenital Heart Diseases, Department of Cardiothoracic Surgery, University Medical Center GroningenDepartment of Medical Biology, Amsterdam Cardiovascular Sciences, Amsterdam University Medical CentresAbstract Few experimental model systems are available for the rare congenital heart diseases of double inlet left ventricle (DILV), a subgroup of univentricular hearts, and excessive trabeculation (ET), or noncompaction. Here, we explore the heart of the axolotl salamander (Ambystoma mexicanum, Shaw 1789) as model system of these diseases. Using micro-echocardiography, we assessed the form and function of the heart of the axolotl, an amphibian, and compared this to human DILV (n = 3). The main finding was that both in the axolotl and DILV, blood flows of disparate oxygen saturation can stay separated in a single ventricle. In the axolotl there is a solitary ventricular inlet and outlet, whereas in DILV there are two separate inlets and outlets. Axolotls had a lower resting heart rate compared to DILV (22 vs. 72 beats per minute), lower ejection fraction (47 vs. 58%), and their oxygen consumption at rest was higher than peak oxygen consumption in DILV (30 vs. 17 ml min−1 kg−1). Concerning the ventricular myocardial organization, histology showed trabeculations in ET (n = 5) are much closer to the normal human setting than to the axolotl setting. We conclude that the axolotl heart resembles some aspects of DILV and ET albeit substantial species differences exist.https://doi.org/10.1038/s41598-022-24442-9 |
| spellingShingle | Sophie Meyer Henrik Lauridsen Kathrine Pedersen Sofie Amalie Andersson Pim van Ooij Tineke Willems Rolf M. F. Berger Tjark Ebels Bjarke Jensen Opportunities and short-comings of the axolotl salamander heart as a model system of human single ventricle and excessive trabeculation Scientific Reports |
| title | Opportunities and short-comings of the axolotl salamander heart as a model system of human single ventricle and excessive trabeculation |
| title_full | Opportunities and short-comings of the axolotl salamander heart as a model system of human single ventricle and excessive trabeculation |
| title_fullStr | Opportunities and short-comings of the axolotl salamander heart as a model system of human single ventricle and excessive trabeculation |
| title_full_unstemmed | Opportunities and short-comings of the axolotl salamander heart as a model system of human single ventricle and excessive trabeculation |
| title_short | Opportunities and short-comings of the axolotl salamander heart as a model system of human single ventricle and excessive trabeculation |
| title_sort | opportunities and short comings of the axolotl salamander heart as a model system of human single ventricle and excessive trabeculation |
| url | https://doi.org/10.1038/s41598-022-24442-9 |
| work_keys_str_mv | AT sophiemeyer opportunitiesandshortcomingsoftheaxolotlsalamanderheartasamodelsystemofhumansingleventricleandexcessivetrabeculation AT henriklauridsen opportunitiesandshortcomingsoftheaxolotlsalamanderheartasamodelsystemofhumansingleventricleandexcessivetrabeculation AT kathrinepedersen opportunitiesandshortcomingsoftheaxolotlsalamanderheartasamodelsystemofhumansingleventricleandexcessivetrabeculation AT sofieamalieandersson opportunitiesandshortcomingsoftheaxolotlsalamanderheartasamodelsystemofhumansingleventricleandexcessivetrabeculation AT pimvanooij opportunitiesandshortcomingsoftheaxolotlsalamanderheartasamodelsystemofhumansingleventricleandexcessivetrabeculation AT tinekewillems opportunitiesandshortcomingsoftheaxolotlsalamanderheartasamodelsystemofhumansingleventricleandexcessivetrabeculation AT rolfmfberger opportunitiesandshortcomingsoftheaxolotlsalamanderheartasamodelsystemofhumansingleventricleandexcessivetrabeculation AT tjarkebels opportunitiesandshortcomingsoftheaxolotlsalamanderheartasamodelsystemofhumansingleventricleandexcessivetrabeculation AT bjarkejensen opportunitiesandshortcomingsoftheaxolotlsalamanderheartasamodelsystemofhumansingleventricleandexcessivetrabeculation |