Mast Cell Tryptase Promotes Airway Remodeling by Inducing Anti-Apoptotic and Cell Growth Properties in Human Alveolar and Bronchial Epithelial Cells
Bronchial and alveolar remodeling and impaired epithelial function are characteristics of chronic respiratory diseases. In these patients, an increased number of mast cells (MCs) positive for serine proteases, tryptase and chymase, infiltrate the epithelium and alveolar parenchyma. However, little i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/10/1439 |
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| author | Frida Berlin Sofia Mogren Camilla Ly Sangeetha Ramu Morten Hvidtfeldt Lena Uller Celeste Porsbjerg Cecilia K. Andersson |
| author_facet | Frida Berlin Sofia Mogren Camilla Ly Sangeetha Ramu Morten Hvidtfeldt Lena Uller Celeste Porsbjerg Cecilia K. Andersson |
| author_sort | Frida Berlin |
| collection | DOAJ |
| description | Bronchial and alveolar remodeling and impaired epithelial function are characteristics of chronic respiratory diseases. In these patients, an increased number of mast cells (MCs) positive for serine proteases, tryptase and chymase, infiltrate the epithelium and alveolar parenchyma. However, little is known regarding the implication of intraepithelial MCs on the local environment, such as epithelial cell function and properties. In this study, we investigated whether MC tryptase is involved in bronchial and alveolar remodeling and the mechanisms of regulation during inflammation. Using novel holographic live cell imaging, we found that MC tryptase enhanced human bronchial and alveolar epithelial cell growth and shortened the cell division intervals. The elevated cell growth induced by tryptase remained in a pro-inflammatory state. Tryptase also increased the expression of the anti-apoptotic protein BIRC3, as well as growth factor release in epithelial cells. Thus, our data imply that the intraepithelial and alveolar MC release of tryptase may play a critical role in disturbing bronchial epithelial and alveolar homeostasis by altering cell growth–death regulation. |
| first_indexed | 2024-03-11T03:50:33Z |
| format | Article |
| id | doaj.art-446aa61587d04f7eb9e80020ecb71d2f |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T03:50:33Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-446aa61587d04f7eb9e80020ecb71d2f2023-11-18T00:53:38ZengMDPI AGCells2073-44092023-05-011210143910.3390/cells12101439Mast Cell Tryptase Promotes Airway Remodeling by Inducing Anti-Apoptotic and Cell Growth Properties in Human Alveolar and Bronchial Epithelial CellsFrida Berlin0Sofia Mogren1Camilla Ly2Sangeetha Ramu3Morten Hvidtfeldt4Lena Uller5Celeste Porsbjerg6Cecilia K. Andersson7Department of Experimental Medical Science, Lund University, 22184 Lund, SwedenDepartment of Experimental Medical Science, Lund University, 22184 Lund, SwedenDepartment of Experimental Medical Science, Lund University, 22184 Lund, SwedenDepartment of Experimental Medical Science, Lund University, 22184 Lund, SwedenDepartment of Respiratory Medicine, Copenhagen University Hospital Bispebjerg, 2400 Copenhagen, DenmarkDepartment of Experimental Medical Science, Lund University, 22184 Lund, SwedenDepartment of Respiratory Medicine, Copenhagen University Hospital Bispebjerg, 2400 Copenhagen, DenmarkDepartment of Experimental Medical Science, Lund University, 22184 Lund, SwedenBronchial and alveolar remodeling and impaired epithelial function are characteristics of chronic respiratory diseases. In these patients, an increased number of mast cells (MCs) positive for serine proteases, tryptase and chymase, infiltrate the epithelium and alveolar parenchyma. However, little is known regarding the implication of intraepithelial MCs on the local environment, such as epithelial cell function and properties. In this study, we investigated whether MC tryptase is involved in bronchial and alveolar remodeling and the mechanisms of regulation during inflammation. Using novel holographic live cell imaging, we found that MC tryptase enhanced human bronchial and alveolar epithelial cell growth and shortened the cell division intervals. The elevated cell growth induced by tryptase remained in a pro-inflammatory state. Tryptase also increased the expression of the anti-apoptotic protein BIRC3, as well as growth factor release in epithelial cells. Thus, our data imply that the intraepithelial and alveolar MC release of tryptase may play a critical role in disturbing bronchial epithelial and alveolar homeostasis by altering cell growth–death regulation.https://www.mdpi.com/2073-4409/12/10/1439mast cellproteasestryptasebronchial epitheliumalveolar epitheliumgrowth factors |
| spellingShingle | Frida Berlin Sofia Mogren Camilla Ly Sangeetha Ramu Morten Hvidtfeldt Lena Uller Celeste Porsbjerg Cecilia K. Andersson Mast Cell Tryptase Promotes Airway Remodeling by Inducing Anti-Apoptotic and Cell Growth Properties in Human Alveolar and Bronchial Epithelial Cells Cells mast cell proteases tryptase bronchial epithelium alveolar epithelium growth factors |
| title | Mast Cell Tryptase Promotes Airway Remodeling by Inducing Anti-Apoptotic and Cell Growth Properties in Human Alveolar and Bronchial Epithelial Cells |
| title_full | Mast Cell Tryptase Promotes Airway Remodeling by Inducing Anti-Apoptotic and Cell Growth Properties in Human Alveolar and Bronchial Epithelial Cells |
| title_fullStr | Mast Cell Tryptase Promotes Airway Remodeling by Inducing Anti-Apoptotic and Cell Growth Properties in Human Alveolar and Bronchial Epithelial Cells |
| title_full_unstemmed | Mast Cell Tryptase Promotes Airway Remodeling by Inducing Anti-Apoptotic and Cell Growth Properties in Human Alveolar and Bronchial Epithelial Cells |
| title_short | Mast Cell Tryptase Promotes Airway Remodeling by Inducing Anti-Apoptotic and Cell Growth Properties in Human Alveolar and Bronchial Epithelial Cells |
| title_sort | mast cell tryptase promotes airway remodeling by inducing anti apoptotic and cell growth properties in human alveolar and bronchial epithelial cells |
| topic | mast cell proteases tryptase bronchial epithelium alveolar epithelium growth factors |
| url | https://www.mdpi.com/2073-4409/12/10/1439 |
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