A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species
Using alkyl halides to tag reactive sulfur species (RSSs) (H<sub>2</sub>S, per/polysulfide, and protein-SSH) is an extensively applied approach. The underlying supposition is that, as with thiols, RSS reacts with alkyl halides via a nucleophilic substitution reaction. We found that this...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/8/1583 |
| _version_ | 1797432540402810880 |
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| author | Xiaohua Wu Yuping Xin Qingda Wang Yongzhen Xia Luying Xun Huaiwei Liu |
| author_facet | Xiaohua Wu Yuping Xin Qingda Wang Yongzhen Xia Luying Xun Huaiwei Liu |
| author_sort | Xiaohua Wu |
| collection | DOAJ |
| description | Using alkyl halides to tag reactive sulfur species (RSSs) (H<sub>2</sub>S, per/polysulfide, and protein-SSH) is an extensively applied approach. The underlying supposition is that, as with thiols, RSS reacts with alkyl halides via a nucleophilic substitution reaction. We found that this supposition is facing a challenge. RSS also initiates a reductive dehalogenation reaction, which generates the reduced unloaded tag and oxidized RSS. Therefore, RSS content in bio-samples might be underestimated, and its species might not be precisely determined when using alkyl halide agents for its analysis. To calculate to the extent of this underestimation, further studies are still required. |
| first_indexed | 2024-03-09T10:02:20Z |
| format | Article |
| id | doaj.art-4473dbc4a2c74effa82ab5c46ab3730c |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T10:02:20Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-4473dbc4a2c74effa82ab5c46ab3730c2023-12-01T23:20:25ZengMDPI AGAntioxidants2076-39212022-08-01118158310.3390/antiox11081583A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur SpeciesXiaohua Wu0Yuping Xin1Qingda Wang2Yongzhen Xia3Luying Xun4Huaiwei Liu5State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266200, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266200, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266200, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266200, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266200, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao 266200, ChinaUsing alkyl halides to tag reactive sulfur species (RSSs) (H<sub>2</sub>S, per/polysulfide, and protein-SSH) is an extensively applied approach. The underlying supposition is that, as with thiols, RSS reacts with alkyl halides via a nucleophilic substitution reaction. We found that this supposition is facing a challenge. RSS also initiates a reductive dehalogenation reaction, which generates the reduced unloaded tag and oxidized RSS. Therefore, RSS content in bio-samples might be underestimated, and its species might not be precisely determined when using alkyl halide agents for its analysis. To calculate to the extent of this underestimation, further studies are still required.https://www.mdpi.com/2076-3921/11/8/1583RSSalkyl halidesmBBrIAB |
| spellingShingle | Xiaohua Wu Yuping Xin Qingda Wang Yongzhen Xia Luying Xun Huaiwei Liu A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species Antioxidants RSS alkyl halides mBBr IAB |
| title | A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species |
| title_full | A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species |
| title_fullStr | A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species |
| title_full_unstemmed | A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species |
| title_short | A Caveat When Using Alkyl Halides as Tagging Agents to Detect/Quantify Reactive Sulfur Species |
| title_sort | caveat when using alkyl halides as tagging agents to detect quantify reactive sulfur species |
| topic | RSS alkyl halides mBBr IAB |
| url | https://www.mdpi.com/2076-3921/11/8/1583 |
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