Utilizing primary HLA-G+ extravillous trophoblasts and HLA-G+ EVT-like cell lines to study maternal-fetal interactions

Utilizing primary HLA-G+ extravillous trophoblasts and HLA-G+ EVT-like cell lines to study maternal-fetal interactions

Summary: Fetal extravillous trophoblasts (EVTs) are the most invasive cells of the placenta and play a key role in modulating maternal immune responses. Here, we present a protocol to purify and culture human leukocyte antigen-G (HLA-G)+ EVTs. We describe steps for tissue dissection, tissue digestio...

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Bibliographic Details
Main Authors: Ira Hamilton, Nadia M. Ikumi, Sarika Kshirsagar, Wendy A. Goodman, Tamara Tilburgs
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Cell culture
Cell isolation
Cell separation/fractionation
Developmental biology
Immunology
Cell Differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723002435

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http://www.sciencedirect.com/science/article/pii/S2666166723002435

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