Comparison of PCR Techniques in Adulteration Identification of Dairy Products
Economic profit-driven food adulteration has become widespread in the dairy industry. One of the most common forms of dairy adulteration is the substitution of low-priced milk for high-priced milk. This has prompted regulatory authorities to focus on various means of authenticity testing. So far, ma...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/13/7/1450 |
| _version_ | 1827734302818828288 |
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| author | Baiyi Li Mingxue Yu Weiping Xu Lu Chen Juan Han |
| author_facet | Baiyi Li Mingxue Yu Weiping Xu Lu Chen Juan Han |
| author_sort | Baiyi Li |
| collection | DOAJ |
| description | Economic profit-driven food adulteration has become widespread in the dairy industry. One of the most common forms of dairy adulteration is the substitution of low-priced milk for high-priced milk. This has prompted regulatory authorities to focus on various means of authenticity testing. So far, many methods have been developed. Since milk adulteration has been upgraded, which has forced the testing methods to meet the needs of detection, which include DNA-based PCR methods. PCR and PCR-derived methods exhibit multiple advantages for authenticity testing, such as high stability, fast speed, and high efficiency, which meet the needs of modern testing. Therefore, it is important to develop rapid, reliable, and inexpensive PCR-based assays for dairy adulteration identification. In order to provide perspectives for improving adulteration identification methods, this review first summarizes the DNA extraction methods, then compares the advantages and disadvantages of various PCR authenticity testing methods, and finally proposes the directions for improving dairy product adulteration identification methods. |
| first_indexed | 2024-03-11T01:23:24Z |
| format | Article |
| id | doaj.art-4493b5937fa1427182446aab9745a384 |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-11T01:23:24Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-4493b5937fa1427182446aab9745a3842023-11-18T17:54:04ZengMDPI AGAgriculture2077-04722023-07-01137145010.3390/agriculture13071450Comparison of PCR Techniques in Adulteration Identification of Dairy ProductsBaiyi Li0Mingxue Yu1Weiping Xu2Lu Chen3Juan Han4Institute of Food and Nutrition Development, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaInstitute of Food and Nutrition Development, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaInstitute of Food and Nutrition Development, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaInstitute of Food and Nutrition Development, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaInstitute of Food and Nutrition Development, Ministry of Agriculture and Rural Affairs, Beijing 100081, ChinaEconomic profit-driven food adulteration has become widespread in the dairy industry. One of the most common forms of dairy adulteration is the substitution of low-priced milk for high-priced milk. This has prompted regulatory authorities to focus on various means of authenticity testing. So far, many methods have been developed. Since milk adulteration has been upgraded, which has forced the testing methods to meet the needs of detection, which include DNA-based PCR methods. PCR and PCR-derived methods exhibit multiple advantages for authenticity testing, such as high stability, fast speed, and high efficiency, which meet the needs of modern testing. Therefore, it is important to develop rapid, reliable, and inexpensive PCR-based assays for dairy adulteration identification. In order to provide perspectives for improving adulteration identification methods, this review first summarizes the DNA extraction methods, then compares the advantages and disadvantages of various PCR authenticity testing methods, and finally proposes the directions for improving dairy product adulteration identification methods.https://www.mdpi.com/2077-0472/13/7/1450PCRadulteration identification methoddairy productsquality controlfood fraud |
| spellingShingle | Baiyi Li Mingxue Yu Weiping Xu Lu Chen Juan Han Comparison of PCR Techniques in Adulteration Identification of Dairy Products Agriculture PCR adulteration identification method dairy products quality control food fraud |
| title | Comparison of PCR Techniques in Adulteration Identification of Dairy Products |
| title_full | Comparison of PCR Techniques in Adulteration Identification of Dairy Products |
| title_fullStr | Comparison of PCR Techniques in Adulteration Identification of Dairy Products |
| title_full_unstemmed | Comparison of PCR Techniques in Adulteration Identification of Dairy Products |
| title_short | Comparison of PCR Techniques in Adulteration Identification of Dairy Products |
| title_sort | comparison of pcr techniques in adulteration identification of dairy products |
| topic | PCR adulteration identification method dairy products quality control food fraud |
| url | https://www.mdpi.com/2077-0472/13/7/1450 |
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