Protocol for single-cell isolation and genome amplification of environmental microbial eukaryotes for genomic analysis

Protocol for single-cell isolation and genome amplification of environmental microbial eukaryotes for genomic analysis

Summary: We describe environmental microbial eukaryotes (EMEs) sample collection, single-cell isolation, lysis, and genome amplification, followed by the rDNA amplification and OTU screening for recovery of high-quality species-specific genomes for de novo assembly. These protocols are part of our p...

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Bibliographic Details
Main Authors: Doina Ciobanu, Sandy Chan, Steven Ahrendt, C. Alisha Quandt, Gerald L. Benny, Matthew E. Smith, Timothy Y. James, Jan-Fang Cheng
Format: Article
Language:English
Published: Elsevier 2022-03-01
Series:STAR Protocols
Subjects:
Sequence analysis
Cell isolation
Single Cell
Flow Cytometry/Mass Cytometry
Genomics
Sequencing
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721006742

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http://www.sciencedirect.com/science/article/pii/S2666166721006742

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