Double-stranded RNA reduction by chaotropic agents during in vitro transcription of messenger RNA

Double-stranded RNA reduction by chaotropic agents during in vitro transcription of messenger RNA

In-vitro-transcribed messenger RNA (mRNA) has recently shown increasing significance in the development of vaccines and therapeutics. Immunogenic double-stranded RNA (dsRNA) is an undesired byproduct formed during in vitro transcription (IVT), and it is challenging to reduce dsRNA byproduct from mRN...

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Bibliographic Details
Main Authors: Xijun Piao, Vibha Yadav, Eddie Wang, Wayne Chang, Lanna Tau, Benjamin E. Lindenmuth, Sharon X. Wang
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
MT: RNA/DNA editing
messenger RNA
double-stranded RNA
in vitro transcription
mRNA purification
chaotropic agents
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253122001974

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http://www.sciencedirect.com/science/article/pii/S2162253122001974

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