Tuning Enzymatically Crosslinked Silk Fibroin Hydrogel Properties for the Development of a Colorectal Cancer Extravasation 3D Model on a Chip

Abstract Microfluidic devices are now the most promising tool to mimic in vivo like scenarios such as tumorigenesis and metastasis due to its ability to more closely mimic cell's natural microenvironment (such as 3D environment and continuous perfusion of nutrients). In this study, the ability...

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Main Authors: Mariana R. Carvalho, Fátima Raquel Maia, Sílvia Vieira, Rui L. Reis, Joaquim M. Oliveira
Format: Article
Language:English
Published: Wiley 2018-06-01
Series:Global Challenges
Subjects:
Online Access:https://doi.org/10.1002/gch2.201700100
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author Mariana R. Carvalho
Fátima Raquel Maia
Sílvia Vieira
Rui L. Reis
Joaquim M. Oliveira
author_facet Mariana R. Carvalho
Fátima Raquel Maia
Sílvia Vieira
Rui L. Reis
Joaquim M. Oliveira
author_sort Mariana R. Carvalho
collection DOAJ
description Abstract Microfluidic devices are now the most promising tool to mimic in vivo like scenarios such as tumorigenesis and metastasis due to its ability to more closely mimic cell's natural microenvironment (such as 3D environment and continuous perfusion of nutrients). In this study, the ability of 2% and 3% enzymatically crosslinked silk fibroin hydrogels with different mechanical properties are tested in terms of colorectal cancer cell migration, under different microenvironments in a 3D dynamic model. Matrigel is used as control. Moreover, a comprehensive comparison between the traditional Boyden chamber assay and the 3D dynamic microfluidic model in terms of colorectal cancer cell migration is presented. The results show profound differences between the two used biomaterials and the two migration models, which are explored in terms of mechanical properties of the hydrogels as well as the intrinsic characteristics of the models. Moreover, the developed 3D dynamic model is validated by demonstrating that hVCAM‐1 plays a major role in the extravasation process, influencing extravasation rate and traveled distance. Furthermore, the developed model enables precise visualization of cancer cell migration within a 3D matrix in response to microenvironmental cues, shedding light on the importance of biophysical properties in cell behavior.
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spelling doaj.art-44a4ca4a3d324f079bad87e73f0b8ab42023-08-14T09:40:50ZengWileyGlobal Challenges2056-66462018-06-0125-6n/an/a10.1002/gch2.201700100Tuning Enzymatically Crosslinked Silk Fibroin Hydrogel Properties for the Development of a Colorectal Cancer Extravasation 3D Model on a ChipMariana R. Carvalho0Fátima Raquel Maia1Sílvia Vieira2Rui L. Reis3Joaquim M. Oliveira43B's Research Group – Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine Avepark 4805‐017 Barco Guimarães Portugal3B's Research Group – Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine Avepark 4805‐017 Barco Guimarães Portugal3B's Research Group – Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine Avepark 4805‐017 Barco Guimarães Portugal3B's Research Group – Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine Avepark 4805‐017 Barco Guimarães Portugal3B's Research Group – Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine Avepark 4805‐017 Barco Guimarães PortugalAbstract Microfluidic devices are now the most promising tool to mimic in vivo like scenarios such as tumorigenesis and metastasis due to its ability to more closely mimic cell's natural microenvironment (such as 3D environment and continuous perfusion of nutrients). In this study, the ability of 2% and 3% enzymatically crosslinked silk fibroin hydrogels with different mechanical properties are tested in terms of colorectal cancer cell migration, under different microenvironments in a 3D dynamic model. Matrigel is used as control. Moreover, a comprehensive comparison between the traditional Boyden chamber assay and the 3D dynamic microfluidic model in terms of colorectal cancer cell migration is presented. The results show profound differences between the two used biomaterials and the two migration models, which are explored in terms of mechanical properties of the hydrogels as well as the intrinsic characteristics of the models. Moreover, the developed 3D dynamic model is validated by demonstrating that hVCAM‐1 plays a major role in the extravasation process, influencing extravasation rate and traveled distance. Furthermore, the developed model enables precise visualization of cancer cell migration within a 3D matrix in response to microenvironmental cues, shedding light on the importance of biophysical properties in cell behavior.https://doi.org/10.1002/gch2.2017001003D modelcolorectal cancerextravasionmicrofluidicssilk
spellingShingle Mariana R. Carvalho
Fátima Raquel Maia
Sílvia Vieira
Rui L. Reis
Joaquim M. Oliveira
Tuning Enzymatically Crosslinked Silk Fibroin Hydrogel Properties for the Development of a Colorectal Cancer Extravasation 3D Model on a Chip
Global Challenges
3D model
colorectal cancer
extravasion
microfluidics
silk
title Tuning Enzymatically Crosslinked Silk Fibroin Hydrogel Properties for the Development of a Colorectal Cancer Extravasation 3D Model on a Chip
title_full Tuning Enzymatically Crosslinked Silk Fibroin Hydrogel Properties for the Development of a Colorectal Cancer Extravasation 3D Model on a Chip
title_fullStr Tuning Enzymatically Crosslinked Silk Fibroin Hydrogel Properties for the Development of a Colorectal Cancer Extravasation 3D Model on a Chip
title_full_unstemmed Tuning Enzymatically Crosslinked Silk Fibroin Hydrogel Properties for the Development of a Colorectal Cancer Extravasation 3D Model on a Chip
title_short Tuning Enzymatically Crosslinked Silk Fibroin Hydrogel Properties for the Development of a Colorectal Cancer Extravasation 3D Model on a Chip
title_sort tuning enzymatically crosslinked silk fibroin hydrogel properties for the development of a colorectal cancer extravasation 3d model on a chip
topic 3D model
colorectal cancer
extravasion
microfluidics
silk
url https://doi.org/10.1002/gch2.201700100
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