Antidiabetic activity of a Flavonoid-Rich extract from flowers of Wisteria sinensis in type 2 diabetic mice via activation of the IRS-1/PI3K/Akt/GLUT4 pathway
The present study aimed to investigate anti-diabetic activities of a flavonoid-rich extract from Wisteria sinensis (WS-FRE). In vitro, WS-FRE significantly promote the translocation of GLUT4 (Glucose transporter 4) to the plasma membrane. Mechanisms analysis revealed that WS-FRE enhanced glucose upt...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-02-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464620305624 |
| _version_ | 1818952118861561856 |
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| author | Yun Huang Tongxi Zhou Yirui Zhang Huiqi Huang Yuanren Ma Chaoqun Wu Qiang Wang Qinxiong Lin Xinzhou Yang Kejian Pang |
| author_facet | Yun Huang Tongxi Zhou Yirui Zhang Huiqi Huang Yuanren Ma Chaoqun Wu Qiang Wang Qinxiong Lin Xinzhou Yang Kejian Pang |
| author_sort | Yun Huang |
| collection | DOAJ |
| description | The present study aimed to investigate anti-diabetic activities of a flavonoid-rich extract from Wisteria sinensis (WS-FRE). In vitro, WS-FRE significantly promote the translocation of GLUT4 (Glucose transporter 4) to the plasma membrane. Mechanisms analysis revealed that WS-FRE enhanced glucose uptake via activation of Akt and GLUT4. In vivo, 4-week treatment of WS-FRE alleviated the typical symptoms including hyperglycemia, glucose intolerance, hyperinsulinemia and dyslipidemia. Histopathological examinations revealed that WS-FRE relieved hepatic steatosis, adipocyte hypertrophy and pancreatic islet lesion to a certain extent in diabetic mice. Western blot analysis indicated that WS-FRE resulted in the suppression of IRS-1 serine phosphorylation and an elevation of PI3K p85, Akt phosphorylation and GLUT4 expression in insulin target tissues, which contributes to improving insulin sensitivity in peripheral tissues and thereby ameliorating glucose metabolism in diabetic mice. Diverse anti-diabetic effects of WS-FRE may be attributed to regulation of GLUT4, IRS-1 serine phosphorylation, PI3K phosphorylation and Akt phosphorylation. |
| first_indexed | 2024-12-20T09:45:18Z |
| format | Article |
| id | doaj.art-44ab62947c2d43efa970dd90d1fac979 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-20T09:45:18Z |
| publishDate | 2021-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-44ab62947c2d43efa970dd90d1fac9792022-12-21T19:44:46ZengElsevierJournal of Functional Foods1756-46462021-02-0177104338Antidiabetic activity of a Flavonoid-Rich extract from flowers of Wisteria sinensis in type 2 diabetic mice via activation of the IRS-1/PI3K/Akt/GLUT4 pathwayYun Huang0Tongxi Zhou1Yirui Zhang2Huiqi Huang3Yuanren Ma4Chaoqun Wu5Qiang Wang6Qinxiong Lin7Xinzhou Yang8Kejian Pang9School of Pharmaceutical Sciences, South-Central University for Nationalities, 182 Min-Zu Road, Wuhan 430074, PR ChinaSchool of Pharmaceutical Sciences, South-Central University for Nationalities, 182 Min-Zu Road, Wuhan 430074, PR ChinaSchool of Pharmaceutical Sciences, South-Central University for Nationalities, 182 Min-Zu Road, Wuhan 430074, PR ChinaSchool of Pharmaceutical Sciences, South-Central University for Nationalities, 182 Min-Zu Road, Wuhan 430074, PR ChinaSchool of Pharmaceutical Sciences, South-Central University for Nationalities, 182 Min-Zu Road, Wuhan 430074, PR ChinaSchool of Pharmaceutical Sciences, South-Central University for Nationalities, 182 Min-Zu Road, Wuhan 430074, PR ChinaSchool of Pharmaceutical Sciences, South-Central University for Nationalities, 182 Min-Zu Road, Wuhan 430074, PR ChinaSchool of Pharmaceutical Sciences, South-Central University for Nationalities, 182 Min-Zu Road, Wuhan 430074, PR ChinaSchool of Pharmaceutical Sciences, South-Central University for Nationalities, 182 Min-Zu Road, Wuhan 430074, PR China; Corresponding authors.Hotian Uygur Pharmaceutical Co., Ltd, Hotian 848200, PR China; Corresponding authors.The present study aimed to investigate anti-diabetic activities of a flavonoid-rich extract from Wisteria sinensis (WS-FRE). In vitro, WS-FRE significantly promote the translocation of GLUT4 (Glucose transporter 4) to the plasma membrane. Mechanisms analysis revealed that WS-FRE enhanced glucose uptake via activation of Akt and GLUT4. In vivo, 4-week treatment of WS-FRE alleviated the typical symptoms including hyperglycemia, glucose intolerance, hyperinsulinemia and dyslipidemia. Histopathological examinations revealed that WS-FRE relieved hepatic steatosis, adipocyte hypertrophy and pancreatic islet lesion to a certain extent in diabetic mice. Western blot analysis indicated that WS-FRE resulted in the suppression of IRS-1 serine phosphorylation and an elevation of PI3K p85, Akt phosphorylation and GLUT4 expression in insulin target tissues, which contributes to improving insulin sensitivity in peripheral tissues and thereby ameliorating glucose metabolism in diabetic mice. Diverse anti-diabetic effects of WS-FRE may be attributed to regulation of GLUT4, IRS-1 serine phosphorylation, PI3K phosphorylation and Akt phosphorylation.http://www.sciencedirect.com/science/article/pii/S1756464620305624Type 2 diabetes mellitusWisteria sinensisGLUT4PI3K/Akt |
| spellingShingle | Yun Huang Tongxi Zhou Yirui Zhang Huiqi Huang Yuanren Ma Chaoqun Wu Qiang Wang Qinxiong Lin Xinzhou Yang Kejian Pang Antidiabetic activity of a Flavonoid-Rich extract from flowers of Wisteria sinensis in type 2 diabetic mice via activation of the IRS-1/PI3K/Akt/GLUT4 pathway Journal of Functional Foods Type 2 diabetes mellitus Wisteria sinensis GLUT4 PI3K/Akt |
| title | Antidiabetic activity of a Flavonoid-Rich extract from flowers of Wisteria sinensis in type 2 diabetic mice via activation of the IRS-1/PI3K/Akt/GLUT4 pathway |
| title_full | Antidiabetic activity of a Flavonoid-Rich extract from flowers of Wisteria sinensis in type 2 diabetic mice via activation of the IRS-1/PI3K/Akt/GLUT4 pathway |
| title_fullStr | Antidiabetic activity of a Flavonoid-Rich extract from flowers of Wisteria sinensis in type 2 diabetic mice via activation of the IRS-1/PI3K/Akt/GLUT4 pathway |
| title_full_unstemmed | Antidiabetic activity of a Flavonoid-Rich extract from flowers of Wisteria sinensis in type 2 diabetic mice via activation of the IRS-1/PI3K/Akt/GLUT4 pathway |
| title_short | Antidiabetic activity of a Flavonoid-Rich extract from flowers of Wisteria sinensis in type 2 diabetic mice via activation of the IRS-1/PI3K/Akt/GLUT4 pathway |
| title_sort | antidiabetic activity of a flavonoid rich extract from flowers of wisteria sinensis in type 2 diabetic mice via activation of the irs 1 pi3k akt glut4 pathway |
| topic | Type 2 diabetes mellitus Wisteria sinensis GLUT4 PI3K/Akt |
| url | http://www.sciencedirect.com/science/article/pii/S1756464620305624 |
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