Longitudinal temperature measurement can determine humane endpoints in BALB/c mouse models of ESKAPEE infection
ABSTRACTAntimicrobial resistance (AMR) is a worldwide problem, which is driving more preclinical research to find new treatments and countermeasures for drug-resistant bacteria. However, translational models in the preclinical space have remained static for years. To improve animal use ethical consi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2023.2186331 |
| _version_ | 1797367187957088256 |
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| author | Randal Scott Dudis Ting Y. Wong Mariel G. Escatte Yonas A. Alamneh Rania Abu-Taleb Wanwen Su Christine Czintos Timothy A. Fitzgerald Yoann Le Breton Daniel V. Zurawski |
| author_facet | Randal Scott Dudis Ting Y. Wong Mariel G. Escatte Yonas A. Alamneh Rania Abu-Taleb Wanwen Su Christine Czintos Timothy A. Fitzgerald Yoann Le Breton Daniel V. Zurawski |
| author_sort | Randal Scott Dudis |
| collection | DOAJ |
| description | ABSTRACTAntimicrobial resistance (AMR) is a worldwide problem, which is driving more preclinical research to find new treatments and countermeasures for drug-resistant bacteria. However, translational models in the preclinical space have remained static for years. To improve animal use ethical considerations, we assessed novel methods to evaluate survival after lethal infection with ESKAPEE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter cloacae, and Escherichia coli) in pulmonary models of infection. Consistent with published lung infection models often used for novel antimicrobial development, BALB/c mice were immunosuppressed with cyclophosphamide and inoculated intranasally with individual ESKAPEE pathogens or sterile saline. Observations were recorded at frequent intervals to determine predictive thresholds for humane endpoint decision-making. Internal temperature was measured via implanted IPTT300 microchips, and external temperature was measured using a non-contact, infrared thermometer. Additionally, clinical scores were evaluated based on animal appearance, behaviour, hydration status, respiration, and body weight. Internal temperature differences between survivors and non-survivors were statistically significant for E. faecium, S. aureus, K. pneumoniae, A. baumannii, E. cloacae, and E. coli, and external temperature differences were statistically significant for S. aureus, K. pneumoniae, E. cloacae, and E. coli. Internal temperature more precisely predicted mortality compared to external temperature, indicating that a threshold of 85ºF (29.4ºC) was 86.0% predictive of mortality and 98.7% predictive of survival. Based on our findings, we recommend future studies involving BALB/c mice ESKAPEE pathogen infection use temperature monitoring as a humane endpoint threshold. |
| first_indexed | 2024-03-08T17:13:41Z |
| format | Article |
| id | doaj.art-44b9872e0c5447949a8b4aa7924ed5c4 |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-03-08T17:13:41Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-44b9872e0c5447949a8b4aa7924ed5c42024-01-03T17:26:57ZengTaylor & Francis GroupVirulence2150-55942150-56082023-12-0114110.1080/21505594.2023.2186331Longitudinal temperature measurement can determine humane endpoints in BALB/c mouse models of ESKAPEE infectionRandal Scott Dudis0Ting Y. Wong1Mariel G. Escatte2Yonas A. Alamneh3Rania Abu-Taleb4Wanwen Su5Christine Czintos6Timothy A. Fitzgerald7Yoann Le Breton8Daniel V. Zurawski9Veterinary Services Program, Center for Enabling Capabilities, Walter Reed Army Institute of Research, Silver Spring, MD, USAWound Infections Department, Bacterial Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USAWound Infections Department, Bacterial Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USAWound Infections Department, Bacterial Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USAWound Infections Department, Bacterial Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USAWound Infections Department, Bacterial Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USAWound Infections Department, Bacterial Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USAWound Infections Department, Bacterial Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USAWound Infections Department, Bacterial Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USAWound Infections Department, Bacterial Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, USAABSTRACTAntimicrobial resistance (AMR) is a worldwide problem, which is driving more preclinical research to find new treatments and countermeasures for drug-resistant bacteria. However, translational models in the preclinical space have remained static for years. To improve animal use ethical considerations, we assessed novel methods to evaluate survival after lethal infection with ESKAPEE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter cloacae, and Escherichia coli) in pulmonary models of infection. Consistent with published lung infection models often used for novel antimicrobial development, BALB/c mice were immunosuppressed with cyclophosphamide and inoculated intranasally with individual ESKAPEE pathogens or sterile saline. Observations were recorded at frequent intervals to determine predictive thresholds for humane endpoint decision-making. Internal temperature was measured via implanted IPTT300 microchips, and external temperature was measured using a non-contact, infrared thermometer. Additionally, clinical scores were evaluated based on animal appearance, behaviour, hydration status, respiration, and body weight. Internal temperature differences between survivors and non-survivors were statistically significant for E. faecium, S. aureus, K. pneumoniae, A. baumannii, E. cloacae, and E. coli, and external temperature differences were statistically significant for S. aureus, K. pneumoniae, E. cloacae, and E. coli. Internal temperature more precisely predicted mortality compared to external temperature, indicating that a threshold of 85ºF (29.4ºC) was 86.0% predictive of mortality and 98.7% predictive of survival. Based on our findings, we recommend future studies involving BALB/c mice ESKAPEE pathogen infection use temperature monitoring as a humane endpoint threshold.https://www.tandfonline.com/doi/10.1080/21505594.2023.2186331TemperatureESKAPEEmurine pulmonary infection modelBALB/chumane endpointsreplacement, reduction, refinement (3Rs) |
| spellingShingle | Randal Scott Dudis Ting Y. Wong Mariel G. Escatte Yonas A. Alamneh Rania Abu-Taleb Wanwen Su Christine Czintos Timothy A. Fitzgerald Yoann Le Breton Daniel V. Zurawski Longitudinal temperature measurement can determine humane endpoints in BALB/c mouse models of ESKAPEE infection Virulence Temperature ESKAPEE murine pulmonary infection model BALB/c humane endpoints replacement, reduction, refinement (3Rs) |
| title | Longitudinal temperature measurement can determine humane endpoints in BALB/c mouse models of ESKAPEE infection |
| title_full | Longitudinal temperature measurement can determine humane endpoints in BALB/c mouse models of ESKAPEE infection |
| title_fullStr | Longitudinal temperature measurement can determine humane endpoints in BALB/c mouse models of ESKAPEE infection |
| title_full_unstemmed | Longitudinal temperature measurement can determine humane endpoints in BALB/c mouse models of ESKAPEE infection |
| title_short | Longitudinal temperature measurement can determine humane endpoints in BALB/c mouse models of ESKAPEE infection |
| title_sort | longitudinal temperature measurement can determine humane endpoints in balb c mouse models of eskapee infection |
| topic | Temperature ESKAPEE murine pulmonary infection model BALB/c humane endpoints replacement, reduction, refinement (3Rs) |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2023.2186331 |
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