A non-canonical nucleophile unlocks a new mechanistic pathway in a designed enzyme
Abstract Directed evolution of computationally designed enzymes has provided new insights into the emergence of sophisticated catalytic sites in proteins. In this regard, we have recently shown that a histidine nucleophile and a flexible arginine can work in synergy to accelerate the Morita-Baylis-H...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2024-03-01
|
Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-024-46123-z |