A DNA Vaccine Encoding the Full-Length Spike Protein of Beta Variant (B.1.351) Elicited Broader Cross-Reactive Immune Responses against Other SARS-CoV-2 Variants
The SARS-CoV-2 pandemic remains an ongoing threat to global health with emerging variants, especially the Omicron variant and its sub-lineages. Although large-scale vaccination worldwide has delivered outstanding achievements for COVID-19 prevention, a declining effectiveness to a different extent i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/11/3/513 |
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| author | Gan Zhao Zhiyu Zhang Yuan Ding Jiawang Hou Ying Liu Mengying Zhang Cheng Sui Limei Wang Xin Xu Xiaoming Gao Zhihua Kou |
| author_facet | Gan Zhao Zhiyu Zhang Yuan Ding Jiawang Hou Ying Liu Mengying Zhang Cheng Sui Limei Wang Xin Xu Xiaoming Gao Zhihua Kou |
| author_sort | Gan Zhao |
| collection | DOAJ |
| description | The SARS-CoV-2 pandemic remains an ongoing threat to global health with emerging variants, especially the Omicron variant and its sub-lineages. Although large-scale vaccination worldwide has delivered outstanding achievements for COVID-19 prevention, a declining effectiveness to a different extent in emerging SARS-CoV-2 variants was observed in the vaccinated population. Vaccines eliciting broader spectrum neutralizing antibodies and cellular immune responses are urgently needed and important. To achieve this goal, rational vaccine design, including antigen modeling, screening and combination, vaccine pipelines, and delivery, are keys to developing a next-generation COVID-19 vaccine. In this study, we designed several DNA constructs based on codon-optimized spike coding regions of several SARS-CoV-2 variants and analyzed their cross-reactive antibodies, including neutralizing antibodies, and cellular immune responses against several VOCs in C57BL/6 mice. The results revealed that different SARS-CoV-2 VOCs induced different cross-reactivity; pBeta, a DNA vaccine encoding the spike protein of the Beta variant, elicited broader cross-reactive neutralizing antibodies against other variants including the Omicron variants BA.1 and BA.4/5. This result demonstrates that the spike antigen from the Beta variant potentially serves as one of the antigens for multivalent vaccine design and development against variants of SARS-CoV-2. |
| first_indexed | 2024-03-11T05:47:57Z |
| format | Article |
| id | doaj.art-44ff8bffe00a481cbf3b196f119d3182 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-11T05:47:57Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-44ff8bffe00a481cbf3b196f119d31822023-11-17T14:17:06ZengMDPI AGVaccines2076-393X2023-02-0111351310.3390/vaccines11030513A DNA Vaccine Encoding the Full-Length Spike Protein of Beta Variant (B.1.351) Elicited Broader Cross-Reactive Immune Responses against Other SARS-CoV-2 VariantsGan Zhao0Zhiyu Zhang1Yuan Ding2Jiawang Hou3Ying Liu4Mengying Zhang5Cheng Sui6Limei Wang7Xin Xu8Xiaoming Gao9Zhihua Kou10Advaccine Biopharmaceutics (Suzhou) Co., Ltd., Suzhou 215000, ChinaAdvaccine Biopharmaceutics (Suzhou) Co., Ltd., Suzhou 215000, ChinaAdvaccine Biopharmaceutics (Suzhou) Co., Ltd., Suzhou 215000, ChinaAdvaccine Biopharmaceutics (Suzhou) Co., Ltd., Suzhou 215000, ChinaAdvaccine Biopharmaceutics (Suzhou) Co., Ltd., Suzhou 215000, ChinaAdvaccine Biopharmaceutics (Suzhou) Co., Ltd., Suzhou 215000, ChinaAdvaccine Biopharmaceutics (Suzhou) Co., Ltd., Suzhou 215000, ChinaAdvaccine Biopharmaceutics (Suzhou) Co., Ltd., Suzhou 215000, ChinaAdvaccine Biopharmaceutics (Suzhou) Co., Ltd., Suzhou 215000, ChinaAdvaccine Biopharmaceutics (Suzhou) Co., Ltd., Suzhou 215000, ChinaAdvaccine Biopharmaceutics (Suzhou) Co., Ltd., Suzhou 215000, ChinaThe SARS-CoV-2 pandemic remains an ongoing threat to global health with emerging variants, especially the Omicron variant and its sub-lineages. Although large-scale vaccination worldwide has delivered outstanding achievements for COVID-19 prevention, a declining effectiveness to a different extent in emerging SARS-CoV-2 variants was observed in the vaccinated population. Vaccines eliciting broader spectrum neutralizing antibodies and cellular immune responses are urgently needed and important. To achieve this goal, rational vaccine design, including antigen modeling, screening and combination, vaccine pipelines, and delivery, are keys to developing a next-generation COVID-19 vaccine. In this study, we designed several DNA constructs based on codon-optimized spike coding regions of several SARS-CoV-2 variants and analyzed their cross-reactive antibodies, including neutralizing antibodies, and cellular immune responses against several VOCs in C57BL/6 mice. The results revealed that different SARS-CoV-2 VOCs induced different cross-reactivity; pBeta, a DNA vaccine encoding the spike protein of the Beta variant, elicited broader cross-reactive neutralizing antibodies against other variants including the Omicron variants BA.1 and BA.4/5. This result demonstrates that the spike antigen from the Beta variant potentially serves as one of the antigens for multivalent vaccine design and development against variants of SARS-CoV-2.https://www.mdpi.com/2076-393X/11/3/513SARS-CoV-2variant of concern (VOC)cross-reactive immune responsesvaccineDNA vaccine |
| spellingShingle | Gan Zhao Zhiyu Zhang Yuan Ding Jiawang Hou Ying Liu Mengying Zhang Cheng Sui Limei Wang Xin Xu Xiaoming Gao Zhihua Kou A DNA Vaccine Encoding the Full-Length Spike Protein of Beta Variant (B.1.351) Elicited Broader Cross-Reactive Immune Responses against Other SARS-CoV-2 Variants Vaccines SARS-CoV-2 variant of concern (VOC) cross-reactive immune responses vaccine DNA vaccine |
| title | A DNA Vaccine Encoding the Full-Length Spike Protein of Beta Variant (B.1.351) Elicited Broader Cross-Reactive Immune Responses against Other SARS-CoV-2 Variants |
| title_full | A DNA Vaccine Encoding the Full-Length Spike Protein of Beta Variant (B.1.351) Elicited Broader Cross-Reactive Immune Responses against Other SARS-CoV-2 Variants |
| title_fullStr | A DNA Vaccine Encoding the Full-Length Spike Protein of Beta Variant (B.1.351) Elicited Broader Cross-Reactive Immune Responses against Other SARS-CoV-2 Variants |
| title_full_unstemmed | A DNA Vaccine Encoding the Full-Length Spike Protein of Beta Variant (B.1.351) Elicited Broader Cross-Reactive Immune Responses against Other SARS-CoV-2 Variants |
| title_short | A DNA Vaccine Encoding the Full-Length Spike Protein of Beta Variant (B.1.351) Elicited Broader Cross-Reactive Immune Responses against Other SARS-CoV-2 Variants |
| title_sort | dna vaccine encoding the full length spike protein of beta variant b 1 351 elicited broader cross reactive immune responses against other sars cov 2 variants |
| topic | SARS-CoV-2 variant of concern (VOC) cross-reactive immune responses vaccine DNA vaccine |
| url | https://www.mdpi.com/2076-393X/11/3/513 |
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