Rapid Multiplexed Flow Cytometric Validation of CRISPRi sgRNAs in Tissue Culture
Genome-wide CRISPR-based screening is a powerful tool in forward genetics, enabling biologic discovery by linking a desired phenotype to a specific genetic perturbation. However, hits from a genome-wide screen require individual validation to reproduce and accurately quantify their effects outside...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bio-protocol LLC
2023-01-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=4591&type=0 |