Single-molecule tracking of PprI in D. radiodurans without interference of autoblinking

Single-molecule tracking of PprI in D. radiodurans without interference of autoblinking

Autoblinking is a widespread phenomenon and exhibits high level of intensity in some bacteria. In Deinococcus radiodurans (D. radiodurans), strong autoblinking was found to be indistinguishable from PAmCherry and greatly prevented single-molecule tracking of proteins of interest. Here we employed th...

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Bibliographic Details
Main Authors: Fanfan Zhai, Li Hao, Xiaomin Chen, Ting Jiang, Qianhong Guo, Liping Xie, Ying Ma, Xiaobo Du, Zhiqin Zheng, Kun Chen, Jun Fan
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-11-01
Series:Frontiers in Microbiology
Subjects:
single-molecule tracking
PprI protein
mMaple3
autoblinking
Deinococcus radiodurans
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2023.1256711/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2023.1256711/full

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