Genes adapt to outsmart gene-targeting strategies in mutant mouse strains by skipping exons to reinitiate transcription and translation

Genes adapt to outsmart gene-targeting strategies in mutant mouse strains by skipping exons to reinitiate transcription and translation

Abstract Background Gene disruption in mouse embryonic stem cells or zygotes is a conventional genetics approach to identify gene function in vivo. However, because different gene disruption strategies use different mechanisms to disrupt genes, the strategies can result in diverse phenotypes in the...

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Bibliographic Details
Main Authors: Vishnu Hosur, Benjamin E. Low, Daniel Li, Grace A. Stafford, Vivek Kohar, Leonard D. Shultz, Michael V. Wiles
Format: Article
Language:English
Published: BMC 2020-07-01
Series:Genome Biology
Subjects:
Definitive-null
Knockout-first
CRISPR/Cas9
Gene disruption
Exon skipping
Gene editing
Online Access:http://link.springer.com/article/10.1186/s13059-020-02086-0

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http://link.springer.com/article/10.1186/s13059-020-02086-0

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