Quantitative detection of the Ralstonia solanacearum species complex in soil by qPCR combined with a recombinant internal control strain

Quantitative detection of the Ralstonia solanacearum species complex in soil by qPCR combined with a recombinant internal control strain

ABSTRACT The detection of soil-borne pathogens by quantitative PCR (qPCR) has been challenging due to the pronounced influence of soil type on DNA extraction and PCR reactions. In the present study, we developed a novel qPCR system and an internal sample process control (ISPC) strain, RsPC, for the...

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Bibliographic Details
Main Authors: Wei Chen, Jun-Wei Zhang, Bi-Xia Qin, Hui-Ting Xie, Zhi Zhang, Xiu-Ze Qiao, Shan-Kui Li, Muhammad Asif, Song Guo, Li-Xian Cui, Pei-Pei Wang, Li-Hong Dong, Qing-Gang Guo, Wen-Jun Jiang, Ping Ma, Zhen-Yuan Xia, Can-Hua Lu, Li-Qun Zhang
Format: Article
Language:English
Published: American Society for Microbiology 2023-12-01
Series:Microbiology Spectrum
Subjects:
Ralstonia solanacearum species complex
quantitative PCR
large-scale sequence analysis
internal sample process control
Online Access:https://journals.asm.org/doi/10.1128/spectrum.00210-23

Internet

https://journals.asm.org/doi/10.1128/spectrum.00210-23

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