txci-ATAC-seq: a massive-scale single-cell technique to profile chromatin accessibility

txci-ATAC-seq: a massive-scale single-cell technique to profile chromatin accessibility

Abstract We develop a large-scale single-cell ATAC-seq method by combining Tn5-based pre-indexing with 10× Genomics barcoding, enabling the indexing of up to 200,000 nuclei across multiple samples in a single reaction. We profile 449,953 nuclei across diverse tissues, including the human cortex, mou...

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Bibliographic Details
Main Authors: Hao Zhang, Ryan M. Mulqueen, Natalie Iannuzo, Dominique O. Farrera, Francesca Polverino, James J. Galligan, Julie G. Ledford, Andrew C. Adey, Darren A. Cusanovich
Format: Article
Language:English
Published: BMC 2024-03-01
Series:Genome Biology
Subjects:
Single-cell
ATAC-seq
Chromatin accessibility
Combinatorial indexing
Molecular hashing
CC16
Online Access:https://doi.org/10.1186/s13059-023-03150-1

Internet

https://doi.org/10.1186/s13059-023-03150-1

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