In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice

In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice

Summary: We present a protocol to prepare mouse cranial window implantation for in vivo two-photon wide-field calcium imaging. This protocol uses G-CaMP9a transgenic mice, which express a genetically encoded calcium indicator with high signal-to-noise ratio. We describe in utero electroporation, fol...

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Bibliographic Details
Main Authors: Masayuki Sakamoto, Keisuke Ota, Yayoi Kondo, Michiko Okamura, Hajime Fujii, Haruhiko Bito
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Developmental biology
Microscopy
Model Organisms
Neuroscience
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672200301X

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http://www.sciencedirect.com/science/article/pii/S266616672200301X

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