In vitro Antimicrobial Potency of Lemon Fruit (Citrus limon) Extract on Salmonella typhi
Introduction: This study aimed to evaluate minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of lemon fruit (Citrus limon) extract in inhibiting Salmonella typhi growth in vitro. Methods: This research was categorized as a laboratory experimental study. Lemon fruit...
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| Format: | Article |
| Language: | English |
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Fakultas Kedokteran Universitas Airlangga
2020-08-01
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| Series: | Juxta: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/JUXTA/article/view/19645 |
| _version_ | 1797954160313761792 |
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| author | Farhan Haidar Fazlur Rahman Lindawati Alimsardjono Sunarni Zakaria |
| author_facet | Farhan Haidar Fazlur Rahman Lindawati Alimsardjono Sunarni Zakaria |
| author_sort | Farhan Haidar Fazlur Rahman |
| collection | DOAJ |
| description | Introduction: This study aimed to evaluate minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of lemon fruit (Citrus limon) extract in inhibiting Salmonella typhi growth in vitro.
Methods: This research was categorized as a laboratory experimental study. Lemon fruit (Citrus limon) extract was prepared with concentration as follows: 100.000 ppm, 50.000 ppm, 25.000 ppm, 12.500 ppm, 6.250 ppm, 3.125 ppm, 1.562 ppm, 781 ppm, and 390 ppm. Dilution tests with Mueller-Hinton broth medium were performed to determine the MIC. After 24 hours of incubation, isolated Salmonella typhi inside the tube was inoculated back in MacConkey agar plate medium to determine the MBC. Replications were conducted 3 times according to Federer’s formula.
Results: MIC of lemon fruit (Citrus limon) extract to Salmonella typhi was determined at 3.125 ppm. Meanwhile, MBC was determined at 6.250 ppm.
Conclusion: This study showed the potential antimicrobial effect of lemon fruit (Citrus limon) extract against Salmonella typhi in-vitro. Further studies are still needed to determine its efficacy and safety in vivo and also its full antibacterial spectrum. |
| first_indexed | 2024-04-10T23:13:13Z |
| format | Article |
| id | doaj.art-45ecfe9438ab4a6ab74e445a30607929 |
| institution | Directory Open Access Journal |
| issn | 1907-3623 2684-9453 |
| language | English |
| last_indexed | 2024-04-10T23:13:13Z |
| publishDate | 2020-08-01 |
| publisher | Fakultas Kedokteran Universitas Airlangga |
| record_format | Article |
| series | Juxta: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga |
| spelling | doaj.art-45ecfe9438ab4a6ab74e445a306079292023-01-13T03:50:21ZengFakultas Kedokteran Universitas AirlanggaJuxta: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga1907-36232684-94532020-08-01112697310.20473/juxta.V11I22020.69-7316057In vitro Antimicrobial Potency of Lemon Fruit (Citrus limon) Extract on Salmonella typhiFarhan Haidar Fazlur Rahman0Lindawati Alimsardjono1Sunarni Zakaria2Universitas AirlanggaUniversitas AirlanggaUniversitas AirlanggaIntroduction: This study aimed to evaluate minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of lemon fruit (Citrus limon) extract in inhibiting Salmonella typhi growth in vitro. Methods: This research was categorized as a laboratory experimental study. Lemon fruit (Citrus limon) extract was prepared with concentration as follows: 100.000 ppm, 50.000 ppm, 25.000 ppm, 12.500 ppm, 6.250 ppm, 3.125 ppm, 1.562 ppm, 781 ppm, and 390 ppm. Dilution tests with Mueller-Hinton broth medium were performed to determine the MIC. After 24 hours of incubation, isolated Salmonella typhi inside the tube was inoculated back in MacConkey agar plate medium to determine the MBC. Replications were conducted 3 times according to Federer’s formula. Results: MIC of lemon fruit (Citrus limon) extract to Salmonella typhi was determined at 3.125 ppm. Meanwhile, MBC was determined at 6.250 ppm. Conclusion: This study showed the potential antimicrobial effect of lemon fruit (Citrus limon) extract against Salmonella typhi in-vitro. Further studies are still needed to determine its efficacy and safety in vivo and also its full antibacterial spectrum.https://e-journal.unair.ac.id/JUXTA/article/view/19645salmonella typhicitrus limonlemonantimicrobialmicmbc |
| spellingShingle | Farhan Haidar Fazlur Rahman Lindawati Alimsardjono Sunarni Zakaria In vitro Antimicrobial Potency of Lemon Fruit (Citrus limon) Extract on Salmonella typhi Juxta: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga salmonella typhi citrus limon lemon antimicrobial mic mbc |
| title | In vitro Antimicrobial Potency of Lemon Fruit (Citrus limon) Extract on Salmonella typhi |
| title_full | In vitro Antimicrobial Potency of Lemon Fruit (Citrus limon) Extract on Salmonella typhi |
| title_fullStr | In vitro Antimicrobial Potency of Lemon Fruit (Citrus limon) Extract on Salmonella typhi |
| title_full_unstemmed | In vitro Antimicrobial Potency of Lemon Fruit (Citrus limon) Extract on Salmonella typhi |
| title_short | In vitro Antimicrobial Potency of Lemon Fruit (Citrus limon) Extract on Salmonella typhi |
| title_sort | in vitro antimicrobial potency of lemon fruit citrus limon extract on salmonella typhi |
| topic | salmonella typhi citrus limon lemon antimicrobial mic mbc |
| url | https://e-journal.unair.ac.id/JUXTA/article/view/19645 |
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