Inhibition of nuclear factor-kappaB, cyclooxygenase-2, and metalloproteinase-9 expression by flavanols from evening primrose (Oenothera paradoxa) in human colon cancer SW-480 cells
The present study examines the chemical composition of an evening primrose flavanol preparation (EPFP) and its chemopreventive activity in colon cancer SW-480 cells. EPFP was found to inhibit SW-480 cell viability by 67% vs. controls at 150 µM catechin equivalents (CE) following 72 h incubation (IC5...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-10-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464617304930 |
| _version_ | 1818596315988230144 |
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| author | Katarzyna Owczarek Elżbieta Hrabec Jakub Fichna Dorota Sosnowska Maria Koziołkiewicz Jacek Szymański Urszula Lewandowska |
| author_facet | Katarzyna Owczarek Elżbieta Hrabec Jakub Fichna Dorota Sosnowska Maria Koziołkiewicz Jacek Szymański Urszula Lewandowska |
| author_sort | Katarzyna Owczarek |
| collection | DOAJ |
| description | The present study examines the chemical composition of an evening primrose flavanol preparation (EPFP) and its chemopreventive activity in colon cancer SW-480 cells. EPFP was found to inhibit SW-480 cell viability by 67% vs. controls at 150 µM catechin equivalents (CE) following 72 h incubation (IC50 = 65 µM CE). EPFP decreased both NF-κB protein expression (33% vs. control at 100 µM CE) and phosphorylation at serine 529 of the p65 subunit (49% vs. control at 100 µM CE) in SW-480 cells. Moreover, 100 µM CE EPFP suppressed COX-2 mRNA expression to 23% of control values and protein expression to 78%. In addition, EPFP reduced MMP-9 protein expression to 24% vs. controls at 100 µM CE. These results provide the first evidence that EPFP reduces NF-κB, COX-2, and MMP-9 expression, which may suggest it has cytotoxic, anti-inflammatory, and anti-metastatic activities towards colon cancer cells. |
| first_indexed | 2024-12-16T11:29:58Z |
| format | Article |
| id | doaj.art-45fd5c22617243cd88d285a93de891e5 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-16T11:29:58Z |
| publishDate | 2017-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-45fd5c22617243cd88d285a93de891e52022-12-21T22:33:15ZengElsevierJournal of Functional Foods1756-46462017-10-0137553563Inhibition of nuclear factor-kappaB, cyclooxygenase-2, and metalloproteinase-9 expression by flavanols from evening primrose (Oenothera paradoxa) in human colon cancer SW-480 cellsKatarzyna Owczarek0Elżbieta Hrabec1Jakub Fichna2Dorota Sosnowska3Maria Koziołkiewicz4Jacek Szymański5Urszula Lewandowska6Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, Lodz, PolandDepartment of Medical Enzymology, Faculty of Medicine, Medical University of Lodz, Lodz, PolandDepartment of Biochemistry, Faculty of Medicine, Medical University of Lodz, Lodz, PolandInstitute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Lodz, PolandInstitute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Lodz, PolandCentral Scientific Laboratory, Faculty of Health Sciences, Medical University of Lodz, Lodz, PolandDepartment of Biochemistry, Faculty of Medicine, Medical University of Lodz, Lodz, Poland; Corresponding author at: Department of Biochemistry, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, Poland.The present study examines the chemical composition of an evening primrose flavanol preparation (EPFP) and its chemopreventive activity in colon cancer SW-480 cells. EPFP was found to inhibit SW-480 cell viability by 67% vs. controls at 150 µM catechin equivalents (CE) following 72 h incubation (IC50 = 65 µM CE). EPFP decreased both NF-κB protein expression (33% vs. control at 100 µM CE) and phosphorylation at serine 529 of the p65 subunit (49% vs. control at 100 µM CE) in SW-480 cells. Moreover, 100 µM CE EPFP suppressed COX-2 mRNA expression to 23% of control values and protein expression to 78%. In addition, EPFP reduced MMP-9 protein expression to 24% vs. controls at 100 µM CE. These results provide the first evidence that EPFP reduces NF-κB, COX-2, and MMP-9 expression, which may suggest it has cytotoxic, anti-inflammatory, and anti-metastatic activities towards colon cancer cells.http://www.sciencedirect.com/science/article/pii/S1756464617304930FlavanolsEvening primroseColon cellsNF-κBCOX-2MMP-9 |
| spellingShingle | Katarzyna Owczarek Elżbieta Hrabec Jakub Fichna Dorota Sosnowska Maria Koziołkiewicz Jacek Szymański Urszula Lewandowska Inhibition of nuclear factor-kappaB, cyclooxygenase-2, and metalloproteinase-9 expression by flavanols from evening primrose (Oenothera paradoxa) in human colon cancer SW-480 cells Journal of Functional Foods Flavanols Evening primrose Colon cells NF-κB COX-2 MMP-9 |
| title | Inhibition of nuclear factor-kappaB, cyclooxygenase-2, and metalloproteinase-9 expression by flavanols from evening primrose (Oenothera paradoxa) in human colon cancer SW-480 cells |
| title_full | Inhibition of nuclear factor-kappaB, cyclooxygenase-2, and metalloproteinase-9 expression by flavanols from evening primrose (Oenothera paradoxa) in human colon cancer SW-480 cells |
| title_fullStr | Inhibition of nuclear factor-kappaB, cyclooxygenase-2, and metalloproteinase-9 expression by flavanols from evening primrose (Oenothera paradoxa) in human colon cancer SW-480 cells |
| title_full_unstemmed | Inhibition of nuclear factor-kappaB, cyclooxygenase-2, and metalloproteinase-9 expression by flavanols from evening primrose (Oenothera paradoxa) in human colon cancer SW-480 cells |
| title_short | Inhibition of nuclear factor-kappaB, cyclooxygenase-2, and metalloproteinase-9 expression by flavanols from evening primrose (Oenothera paradoxa) in human colon cancer SW-480 cells |
| title_sort | inhibition of nuclear factor kappab cyclooxygenase 2 and metalloproteinase 9 expression by flavanols from evening primrose oenothera paradoxa in human colon cancer sw 480 cells |
| topic | Flavanols Evening primrose Colon cells NF-κB COX-2 MMP-9 |
| url | http://www.sciencedirect.com/science/article/pii/S1756464617304930 |
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