Yeast lacking the sterol C-5 desaturase Erg3 are tolerant to the anti-inflammatory triterpenoid saponin escin
Abstract Escin is a mixture of over 30 glycosylated triterpenoid (saponin) structures, extracted from the dried fruit of horse chestnuts. Escin is currently used as an anti-inflammatory, and has potential applications in the treatment of arthritis and cancer. Engineered yeast would enable production...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-40308-0 |
| _version_ | 1797453126373998592 |
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| author | Emily J. Johnston Jess Tallis Edward Cunningham-Oakes Tessa Moses Simon J. Moore Sarah Hosking Susan J. Rosser |
| author_facet | Emily J. Johnston Jess Tallis Edward Cunningham-Oakes Tessa Moses Simon J. Moore Sarah Hosking Susan J. Rosser |
| author_sort | Emily J. Johnston |
| collection | DOAJ |
| description | Abstract Escin is a mixture of over 30 glycosylated triterpenoid (saponin) structures, extracted from the dried fruit of horse chestnuts. Escin is currently used as an anti-inflammatory, and has potential applications in the treatment of arthritis and cancer. Engineered yeast would enable production of specific bioactive components of escin at industrial scale, however many saponins have been shown to be toxic to yeast. Here we report that a Saccharomyces cerevisiae strain specifically lacking the sterol C-5 desaturase gene ERG3, exhibits striking enhanced tolerance to escin treatment. Transcriptome analyses, as well as pre-mixing of escin with sterols, support the hypothesis that escin interacts directly with ergosterol, but not as strongly with the altered sterols present in erg3Δ. A diverse range of saponins are of commercial interest, and this research highlights the value of screening lipidome mutants to identify appropriate hosts for engineering the industrial production of saponins. |
| first_indexed | 2024-03-09T15:18:24Z |
| format | Article |
| id | doaj.art-45fe8550be604c0293f915b1a5d6929d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:18:24Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-45fe8550be604c0293f915b1a5d6929d2023-11-26T12:56:00ZengNature PortfolioScientific Reports2045-23222023-08-0113111110.1038/s41598-023-40308-0Yeast lacking the sterol C-5 desaturase Erg3 are tolerant to the anti-inflammatory triterpenoid saponin escinEmily J. Johnston0Jess Tallis1Edward Cunningham-Oakes2Tessa Moses3Simon J. Moore4Sarah Hosking5Susan J. Rosser6Centre for Engineering Biology, University of EdinburghCentre for Engineering Biology, University of EdinburghDepartment of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of LiverpoolEdinOmics, RRID:SCR_021838, University of EdinburghGenetic Science Division, Thermo Fisher ScientificUnilever R&D Port SunlightCentre for Engineering Biology, University of EdinburghAbstract Escin is a mixture of over 30 glycosylated triterpenoid (saponin) structures, extracted from the dried fruit of horse chestnuts. Escin is currently used as an anti-inflammatory, and has potential applications in the treatment of arthritis and cancer. Engineered yeast would enable production of specific bioactive components of escin at industrial scale, however many saponins have been shown to be toxic to yeast. Here we report that a Saccharomyces cerevisiae strain specifically lacking the sterol C-5 desaturase gene ERG3, exhibits striking enhanced tolerance to escin treatment. Transcriptome analyses, as well as pre-mixing of escin with sterols, support the hypothesis that escin interacts directly with ergosterol, but not as strongly with the altered sterols present in erg3Δ. A diverse range of saponins are of commercial interest, and this research highlights the value of screening lipidome mutants to identify appropriate hosts for engineering the industrial production of saponins.https://doi.org/10.1038/s41598-023-40308-0 |
| spellingShingle | Emily J. Johnston Jess Tallis Edward Cunningham-Oakes Tessa Moses Simon J. Moore Sarah Hosking Susan J. Rosser Yeast lacking the sterol C-5 desaturase Erg3 are tolerant to the anti-inflammatory triterpenoid saponin escin Scientific Reports |
| title | Yeast lacking the sterol C-5 desaturase Erg3 are tolerant to the anti-inflammatory triterpenoid saponin escin |
| title_full | Yeast lacking the sterol C-5 desaturase Erg3 are tolerant to the anti-inflammatory triterpenoid saponin escin |
| title_fullStr | Yeast lacking the sterol C-5 desaturase Erg3 are tolerant to the anti-inflammatory triterpenoid saponin escin |
| title_full_unstemmed | Yeast lacking the sterol C-5 desaturase Erg3 are tolerant to the anti-inflammatory triterpenoid saponin escin |
| title_short | Yeast lacking the sterol C-5 desaturase Erg3 are tolerant to the anti-inflammatory triterpenoid saponin escin |
| title_sort | yeast lacking the sterol c 5 desaturase erg3 are tolerant to the anti inflammatory triterpenoid saponin escin |
| url | https://doi.org/10.1038/s41598-023-40308-0 |
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