Data-driven identification of total RNA expression genes for estimation of RNA abundance in heterogeneous cell types highlighted in brain tissue

Data-driven identification of total RNA expression genes for estimation of RNA abundance in heterogeneous cell types highlighted in brain tissue

Abstract We define and identify a new class of control genes for next-generation sequencing called total RNA expression genes (TREGs), which correlate with total RNA abundance in cell types of different sizes and transcriptional activity. We provide a data-driven method to identify TREGs from single...

Full description

Bibliographic Details
Main Authors: Louise A. Huuki-Myers, Kelsey D. Montgomery, Sang Ho Kwon, Stephanie C. Page, Stephanie C. Hicks, Kristen R. Maynard, Leonardo Collado-Torres
Format: Article
Language:English
Published: BMC 2023-10-01
Series:Genome Biology
Subjects:
RNA abundance
RNAscope
snRNA-seq
TREG
Deconvolution
Bioconductor
Online Access:https://doi.org/10.1186/s13059-023-03066-w

Internet

https://doi.org/10.1186/s13059-023-03066-w

Similar Items