Direct Lysis RT-qPCR of SARS-CoV-2 in Cell Culture Supernatant Allows for Fast and Accurate Quantification

Direct Lysis RT-qPCR of SARS-CoV-2 in Cell Culture Supernatant Allows for Fast and Accurate Quantification

Studying the entire virus replication cycle of SARS-CoV-2 is essential to identify the host factors involved and treatments to combat infection. Quantification of released virions often requires lengthy procedures, whereas quantification of viral RNA in supernatant is faster and applicable to clinic...

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Bibliographic Details
Main Authors: Nicky Craig, Sarah L. Fletcher, Alison Daniels, Caitlin Newman, Marie O’Shea, Wenfang Spring Tan, Amanda Warr, Christine Tait-Burkard
Format: Article
Language:English
Published: MDPI AG 2022-02-01
Series:Viruses
Subjects:
SARS-CoV-2
real-time PCR
COVID-19
direct lysis
diagnostics
coronavirus
Online Access:https://www.mdpi.com/1999-4915/14/3/508

Internet

https://www.mdpi.com/1999-4915/14/3/508

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