Efficient and versatile multiplex prime editing in hexaploid wheat

Efficient and versatile multiplex prime editing in hexaploid wheat

Abstract Prime editing is limited by low efficiency in plants. Here, we develop an upgraded engineered plant prime editor in hexaploid wheat, ePPEplus, by introducing a V223A substitution into reverse transcriptase in the ePPEmax* architecture. ePPEplus enhances the efficiency by an average 33.0-fol...

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Bibliographic Details
Main Authors: Pei Ni, Yidi Zhao, Ximeng Zhou, Zehua Liu, Zhengwei Huang, Zhongfu Ni, Qixin Sun, Yuan Zong
Format: Article
Language:English
Published: BMC 2023-06-01
Series:Genome Biology
Subjects:
Hexaploid wheat
Prime editing
Reverse transcriptase
Multiplex genome editing
Online Access:https://doi.org/10.1186/s13059-023-02990-1

Internet

https://doi.org/10.1186/s13059-023-02990-1

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