2-Ketogluconate Kinase from <i>Cupriavidus</i> <i>necator</i> H16: Purification, Characterization, and Exploration of Its Substrate Specificity

We have cloned, overexpressed, purified, and characterized a 2-ketogluconate kinase (2-dehydrogluconokinase, EC 2.7.1.13) from <i>Cupriavidus necator (Ralstonia eutropha)</i> H16. Exploration of its substrate specificity revealed that three ketoacids (2-keto-3-deoxy-<span style="...

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Bibliographic Details
Main Authors: Israel Sánchez-Moreno, Natalia Trachtmann, Sibel Ilhan, Virgil Hélaine, Marielle Lemaire, Christine Guérard-Hélaine, Georg A. Sprenger
Format: Article
Language:English
Published: MDPI AG 2019-06-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/24/13/2393