2-Ketogluconate Kinase from <i>Cupriavidus</i> <i>necator</i> H16: Purification, Characterization, and Exploration of Its Substrate Specificity

2-Ketogluconate Kinase from <i>Cupriavidus</i> <i>necator</i> H16: Purification, Characterization, and Exploration of Its Substrate Specificity

We have cloned, overexpressed, purified, and characterized a 2-ketogluconate kinase (2-dehydrogluconokinase, EC 2.7.1.13) from <i>Cupriavidus necator (Ralstonia eutropha)</i> H16. Exploration of its substrate specificity revealed that three ketoacids (2-keto-3-deoxy-<span style="...

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Bibliographic Details
Main Authors:	Israel Sánchez-Moreno, Natalia Trachtmann, Sibel Ilhan, Virgil Hélaine, Marielle Lemaire, Christine Guérard-Hélaine, Georg A. Sprenger
Format:	Article
Language:	English
Published:	MDPI AG 2019-06-01
Series:	Molecules
Subjects:	2-ketogluconate 2-ketogluconate kinase 2-ketogulonate 2-keto-3-deoxygluconate <i>Cupriavidus necator</i> biocatalysis monosaccharides phosphate
Online Access:	https://www.mdpi.com/1420-3049/24/13/2393

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