Detection of Calmodulin-Binding Proteins Using a 32P-Labeled GST-Calmodulin Fusion Protein and a Novel Renaturation Protocol
To identify calmodulin-binding proteins in cellular extracts and tissue homogenates and to analyze purified calmodulin target proteins, overlay procedures using 125I-calmodulin or, more recently, nonradioactive biotinylated calmodulin have been widely used. Here we describe a rapid, alternative meth...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-08-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96212rr03 |