The main protease 3CLpro of the SARS-CoV-2 virus: how to turn an enemy into a helper

The main protease 3CLpro of the SARS-CoV-2 virus: how to turn an enemy into a helper

Despite the long history of use and the knowledge of the genetics and biochemistry of E. coli, problems are still possible in obtaining a soluble form of recombinant proteins in this system. Although, soluble protein can be obtained both in the cytoplasm and in the periplasm of the bacterial cell. T...

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Bibliographic Details
Main Authors: Svetlana V. Belenkaya, Iuliia A. Merkuleva, Olga I. Yarovaya, Varvara Yu. Chirkova, Elena A. Sharlaeva, Daniil V. Shanshin, Ekaterina A. Volosnikova, Sergey Z. Vatsadze, Mikhail V. Khvostov, Nariman F. Salakhutdinov, Dmitriy N. Shcherbakov
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-06-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
E. coli bacteria
3CL protease
TEV protease
RBD
SARS-CoV-2
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2023.1187761/full

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https://www.frontiersin.org/articles/10.3389/fbioe.2023.1187761/full

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