Following Enzyme Activity with Infrared Spectroscopy
Fourier transform infrared (FTIR) spectroscopy provides a direct, "on-line" monitor of enzymatic reactions. Measurement of enzymatic activity is based on the fact that the infrared spectra of reactants and products of an enzymatic reaction are usually different. Several examples are given...
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| Format: | Article |
| Language: | English |
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MDPI AG
2010-03-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/10/4/2626/ |
| _version_ | 1811278224214196224 |
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| author | Saroj Kumar Andreas Barth |
| author_facet | Saroj Kumar Andreas Barth |
| author_sort | Saroj Kumar |
| collection | DOAJ |
| description | Fourier transform infrared (FTIR) spectroscopy provides a direct, "on-line" monitor of enzymatic reactions. Measurement of enzymatic activity is based on the fact that the infrared spectra of reactants and products of an enzymatic reaction are usually different. Several examples are given using the enzymes pyruvate kinase, fumarase and alcohol dehydrogenase. The main advantage of the infrared method is that it observes the reaction of interest directly, i.e.,no activity assay is required to convert the progress of the reaction into an observable quantity. |
| first_indexed | 2024-04-13T00:32:08Z |
| format | Article |
| id | doaj.art-47028e81774f46daa4b73b393d981d3b |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-13T00:32:08Z |
| publishDate | 2010-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-47028e81774f46daa4b73b393d981d3b2022-12-22T03:10:27ZengMDPI AGSensors1424-82202010-03-011042626263710.3390/s100402626Following Enzyme Activity with Infrared SpectroscopySaroj KumarAndreas BarthFourier transform infrared (FTIR) spectroscopy provides a direct, "on-line" monitor of enzymatic reactions. Measurement of enzymatic activity is based on the fact that the infrared spectra of reactants and products of an enzymatic reaction are usually different. Several examples are given using the enzymes pyruvate kinase, fumarase and alcohol dehydrogenase. The main advantage of the infrared method is that it observes the reaction of interest directly, i.e.,no activity assay is required to convert the progress of the reaction into an observable quantity.http://www.mdpi.com/1424-8220/10/4/2626/vibrational spectroscopyinfrared spectroscopyATRFTIRenzyme activity |
| spellingShingle | Saroj Kumar Andreas Barth Following Enzyme Activity with Infrared Spectroscopy Sensors vibrational spectroscopy infrared spectroscopy ATR FTIR enzyme activity |
| title | Following Enzyme Activity with Infrared Spectroscopy |
| title_full | Following Enzyme Activity with Infrared Spectroscopy |
| title_fullStr | Following Enzyme Activity with Infrared Spectroscopy |
| title_full_unstemmed | Following Enzyme Activity with Infrared Spectroscopy |
| title_short | Following Enzyme Activity with Infrared Spectroscopy |
| title_sort | following enzyme activity with infrared spectroscopy |
| topic | vibrational spectroscopy infrared spectroscopy ATR FTIR enzyme activity |
| url | http://www.mdpi.com/1424-8220/10/4/2626/ |
| work_keys_str_mv | AT sarojkumar followingenzymeactivitywithinfraredspectroscopy AT andreasbarth followingenzymeactivitywithinfraredspectroscopy |