Localization of the cannabinoid CB1 receptor and the 2-AG synthesizing (DAGLα) and degrading (MAGL, FAAH) enzymes in cells expressing the Ca2+-binding proteins calbindin, calretinin and parvalbumin in the adult rat hippocampus
The retrograde suppression of the synaptic transmission by the endocannabinoid sn-2-arachidonoylglycerol (2-AG) is mediated by the cannabinoid CB1 receptors and requires the elevation of intracellular Ca2+ and the activation of specific 2-AG synthesizing (i.e. DAGLα) enzymes. However, the anatomical...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-06-01
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| Series: | Frontiers in Neuroanatomy |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnana.2014.00056/full |
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| author | Patricia eRivera Sergio eArrabal Manuel eCifuentes Jesús M. Grondona Margarita ePérez-Martín Leticia eRubio Antonio eVargas Fuentes Antonia eSerrano Francisco Javier ePavon Juan eSuarez Fernando eRodríguez de Fonseca |
| author_facet | Patricia eRivera Sergio eArrabal Manuel eCifuentes Jesús M. Grondona Margarita ePérez-Martín Leticia eRubio Antonio eVargas Fuentes Antonia eSerrano Francisco Javier ePavon Juan eSuarez Fernando eRodríguez de Fonseca |
| author_sort | Patricia eRivera |
| collection | DOAJ |
| description | The retrograde suppression of the synaptic transmission by the endocannabinoid sn-2-arachidonoylglycerol (2-AG) is mediated by the cannabinoid CB1 receptors and requires the elevation of intracellular Ca2+ and the activation of specific 2-AG synthesizing (i.e. DAGLα) enzymes. However, the anatomical organization of the neuronal substrates that express 2-AG/CB1 signaling system-related molecules associated with selective Ca2+-binding proteins (CaBPs) is still unknown. For this purpose, we used double-label immunofluorescence and confocal laser scanning microscopy for the characterization of the expression of the 2-AG/CB1 signaling system (CB1 receptor, DAGLα, MAGL and FAAH) and the CaBPs calbindin D28k, calretinin and parvalbumin in the rat hippocampus. CB1, DAGLα and MAGL labeling was mainly localized in fibers and neuropil, which were differentially organized depending on the hippocampal CaBPs-expressing cells. CB1+ fiber terminals localized in all hippocampal principal cell layers were tightly attached to calbindin+ cells (granular and pyramidal neurons), and calretinin+ and parvalbumin+ interneurons. DAGLα neuropil labeling was selectively found surrounding calbindin+ principal cells in the dentate gyrus and CA1, and in the calretinin+ and parvalbumin+ interneurons in the pyramidal cell layers of the CA1/3 fields. MAGL+ terminals were only observed around CA1 calbindin+ pyramidal cells, CA1/3 calretinin+ interneurons and CA3 parvalbumin+ interneurons localized in the pyramidal cell layers. Interestingly, calbindin+ pyramidal cells expressed FAAH specifically in the CA1 field. The identification of anatomically related-neuronal substrates that expressed 2-AG/CB1 signaling system and selective CaBPs should be considered when analyzing the cannabinoid signaling associated with hippocampal functions. |
| first_indexed | 2024-04-13T23:12:55Z |
| format | Article |
| id | doaj.art-4742e1d5339b45c49e0f3872102d78eb |
| institution | Directory Open Access Journal |
| issn | 1662-5129 |
| language | English |
| last_indexed | 2024-04-13T23:12:55Z |
| publishDate | 2014-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj.art-4742e1d5339b45c49e0f3872102d78eb2022-12-22T02:25:30ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292014-06-01810.3389/fnana.2014.0005689321Localization of the cannabinoid CB1 receptor and the 2-AG synthesizing (DAGLα) and degrading (MAGL, FAAH) enzymes in cells expressing the Ca2+-binding proteins calbindin, calretinin and parvalbumin in the adult rat hippocampusPatricia eRivera0Sergio eArrabal1Manuel eCifuentes2Jesús M. Grondona3Margarita ePérez-Martín4Leticia eRubio5Antonio eVargas Fuentes6Antonia eSerrano7Francisco Javier ePavon8Juan eSuarez9Fernando eRodríguez de Fonseca10IBIMA. Hospital Regional Universitario de Málaga (FIMABIS)IBIMA. Hospital Regional Universitario de Málaga (FIMABIS)Universidad de Málaga (IBIMA)Universidad de Málaga (IBIMA)Universidad de Málaga (IBIMA)Universidad de MálagaIBIMA. Hospital Regional Universitario de Málaga (FIMABIS)IBIMA. Hospital Regional Universitario de Málaga (FIMABIS)IBIMA. Hospital Regional Universitario de Málaga (FIMABIS)IBIMA. Hospital Regional Universitario de Málaga (FIMABIS)IBIMA. Hospital Regional Universitario de Málaga (FIMABIS)The retrograde suppression of the synaptic transmission by the endocannabinoid sn-2-arachidonoylglycerol (2-AG) is mediated by the cannabinoid CB1 receptors and requires the elevation of intracellular Ca2+ and the activation of specific 2-AG synthesizing (i.e. DAGLα) enzymes. However, the anatomical organization of the neuronal substrates that express 2-AG/CB1 signaling system-related molecules associated with selective Ca2+-binding proteins (CaBPs) is still unknown. For this purpose, we used double-label immunofluorescence and confocal laser scanning microscopy for the characterization of the expression of the 2-AG/CB1 signaling system (CB1 receptor, DAGLα, MAGL and FAAH) and the CaBPs calbindin D28k, calretinin and parvalbumin in the rat hippocampus. CB1, DAGLα and MAGL labeling was mainly localized in fibers and neuropil, which were differentially organized depending on the hippocampal CaBPs-expressing cells. CB1+ fiber terminals localized in all hippocampal principal cell layers were tightly attached to calbindin+ cells (granular and pyramidal neurons), and calretinin+ and parvalbumin+ interneurons. DAGLα neuropil labeling was selectively found surrounding calbindin+ principal cells in the dentate gyrus and CA1, and in the calretinin+ and parvalbumin+ interneurons in the pyramidal cell layers of the CA1/3 fields. MAGL+ terminals were only observed around CA1 calbindin+ pyramidal cells, CA1/3 calretinin+ interneurons and CA3 parvalbumin+ interneurons localized in the pyramidal cell layers. Interestingly, calbindin+ pyramidal cells expressed FAAH specifically in the CA1 field. The identification of anatomically related-neuronal substrates that expressed 2-AG/CB1 signaling system and selective CaBPs should be considered when analyzing the cannabinoid signaling associated with hippocampal functions.http://journal.frontiersin.org/Journal/10.3389/fnana.2014.00056/fullHippocampusImmunohistochemistryCannabinoid receptorconfocal microscopyrat2-Arachidonoylglycerol |
| spellingShingle | Patricia eRivera Sergio eArrabal Manuel eCifuentes Jesús M. Grondona Margarita ePérez-Martín Leticia eRubio Antonio eVargas Fuentes Antonia eSerrano Francisco Javier ePavon Juan eSuarez Fernando eRodríguez de Fonseca Localization of the cannabinoid CB1 receptor and the 2-AG synthesizing (DAGLα) and degrading (MAGL, FAAH) enzymes in cells expressing the Ca2+-binding proteins calbindin, calretinin and parvalbumin in the adult rat hippocampus Frontiers in Neuroanatomy Hippocampus Immunohistochemistry Cannabinoid receptor confocal microscopy rat 2-Arachidonoylglycerol |
| title | Localization of the cannabinoid CB1 receptor and the 2-AG synthesizing (DAGLα) and degrading (MAGL, FAAH) enzymes in cells expressing the Ca2+-binding proteins calbindin, calretinin and parvalbumin in the adult rat hippocampus |
| title_full | Localization of the cannabinoid CB1 receptor and the 2-AG synthesizing (DAGLα) and degrading (MAGL, FAAH) enzymes in cells expressing the Ca2+-binding proteins calbindin, calretinin and parvalbumin in the adult rat hippocampus |
| title_fullStr | Localization of the cannabinoid CB1 receptor and the 2-AG synthesizing (DAGLα) and degrading (MAGL, FAAH) enzymes in cells expressing the Ca2+-binding proteins calbindin, calretinin and parvalbumin in the adult rat hippocampus |
| title_full_unstemmed | Localization of the cannabinoid CB1 receptor and the 2-AG synthesizing (DAGLα) and degrading (MAGL, FAAH) enzymes in cells expressing the Ca2+-binding proteins calbindin, calretinin and parvalbumin in the adult rat hippocampus |
| title_short | Localization of the cannabinoid CB1 receptor and the 2-AG synthesizing (DAGLα) and degrading (MAGL, FAAH) enzymes in cells expressing the Ca2+-binding proteins calbindin, calretinin and parvalbumin in the adult rat hippocampus |
| title_sort | localization of the cannabinoid cb1 receptor and the 2 ag synthesizing daglα and degrading magl faah enzymes in cells expressing the ca2 binding proteins calbindin calretinin and parvalbumin in the adult rat hippocampus |
| topic | Hippocampus Immunohistochemistry Cannabinoid receptor confocal microscopy rat 2-Arachidonoylglycerol |
| url | http://journal.frontiersin.org/Journal/10.3389/fnana.2014.00056/full |
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