ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance

Summary: In the field of epigenetic inheritance, delineating molecular mechanisms implicated in the transfer of paternal environmental conditions to descendants has been elusive. This protocol details how to track sperm chromatin intergenerationally. We describe mouse model design to probe chromatin...

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Bibliographic Details
Main Authors: Ariane Lismer, Romain Lambrot, Christine Lafleur, Vanessa Dumeaux, Sarah Kimmins
Format: Article
Language:English
Published: Elsevier 2021-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003099