In Vitro and In Vivo Antidiabetic Activity, Phenolic Content and Microscopical Characterization of <i>Terfezia claveryi</i>
<i>Terfezia claveryi</i> (<i>T. claveryi</i>) is used by traditional healers in the Middle East region to treat several diseases, including diabetes. The present study evaluated the total phenolic and investigated the blood-glucose-lowering potential of different aqueous extr...
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MDPI AG
2022-07-01
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| author | Ala’ Abu-Odeh Mayadah Shehadeh Ghadeer A. R. Y. Suaifan Nida Karameh Diana Abdel Rahman Yasser Kandil |
| author_facet | Ala’ Abu-Odeh Mayadah Shehadeh Ghadeer A. R. Y. Suaifan Nida Karameh Diana Abdel Rahman Yasser Kandil |
| author_sort | Ala’ Abu-Odeh |
| collection | DOAJ |
| description | <i>Terfezia claveryi</i> (<i>T. claveryi</i>) is used by traditional healers in the Middle East region to treat several diseases, including diabetes. The present study evaluated the total phenolic and investigated the blood-glucose-lowering potential of different aqueous extracts of this selected truffle using in vitro and in vivo models. The phytochemical profile was examined using UPLC-MS. The macerate and the microwave-assisted extract were the richest in phenolic compounds. All <i>T. claveryi</i> extracts exhibited a remarkable α-glucosidase inhibitory effect in vitro, with an IC<sub>50</sub> of 2.43, 3.26, 5.18 and 3.31 mg/mL for the aqueous microwave-assisted extract macerate, infusion and decoction, respectively. On the other hand, in the high-fat diet alloxan-induced diabetic mice model, all tested crude aqueous extracts exhibited a significant antihyperglycemic activity (<i>p</i> < 0.05). Four hours after the administration of the 250 mg/kg dose, the macerate was able to induce a 29.4% blood-glucose-lowering effect compared to a 24.8% reduction induced by the infusion, which was sustained for a further two hours. The hypoglycemic effect (29.3% and 32.4%) was also recorded six hours after the administration of the single dose 500 mg/kg of the macerate and the infusion, respectively. Truffle extracts exhibited antidiabetic activity both in vitro and in vivo, providing a rationale for the traditional use as a natural hypoglycemic. |
| first_indexed | 2024-03-09T05:09:55Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-09T05:09:55Z |
| publishDate | 2022-07-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-47518e80eb814b04a5eab0f1937d30532023-12-03T12:49:58ZengMDPI AGMolecules1420-30492022-07-012715484310.3390/molecules27154843In Vitro and In Vivo Antidiabetic Activity, Phenolic Content and Microscopical Characterization of <i>Terfezia claveryi</i>Ala’ Abu-Odeh0Mayadah Shehadeh1Ghadeer A. R. Y. Suaifan2Nida Karameh3Diana Abdel Rahman4Yasser Kandil5Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmacy, Applied Science Private University, Amman 11931, JordanDepartment of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman 11942, JordanDepartment of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman 11942, JordanPharmacological and Diagnostic Research Center (PDRC), Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, JordanDepartment of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman 11942, JordanDepartment of Biochemistry and Molecular Biology, Faculty of Pharmacy, Al-Azhar University, Cairo 4434004, Egypt<i>Terfezia claveryi</i> (<i>T. claveryi</i>) is used by traditional healers in the Middle East region to treat several diseases, including diabetes. The present study evaluated the total phenolic and investigated the blood-glucose-lowering potential of different aqueous extracts of this selected truffle using in vitro and in vivo models. The phytochemical profile was examined using UPLC-MS. The macerate and the microwave-assisted extract were the richest in phenolic compounds. All <i>T. claveryi</i> extracts exhibited a remarkable α-glucosidase inhibitory effect in vitro, with an IC<sub>50</sub> of 2.43, 3.26, 5.18 and 3.31 mg/mL for the aqueous microwave-assisted extract macerate, infusion and decoction, respectively. On the other hand, in the high-fat diet alloxan-induced diabetic mice model, all tested crude aqueous extracts exhibited a significant antihyperglycemic activity (<i>p</i> < 0.05). Four hours after the administration of the 250 mg/kg dose, the macerate was able to induce a 29.4% blood-glucose-lowering effect compared to a 24.8% reduction induced by the infusion, which was sustained for a further two hours. The hypoglycemic effect (29.3% and 32.4%) was also recorded six hours after the administration of the single dose 500 mg/kg of the macerate and the infusion, respectively. Truffle extracts exhibited antidiabetic activity both in vitro and in vivo, providing a rationale for the traditional use as a natural hypoglycemic.https://www.mdpi.com/1420-3049/27/15/4843diabetestruffle<i>Terfezia claveryi</i>total phenolsα-glucosidase inhibitionantihyperglycemic |
| spellingShingle | Ala’ Abu-Odeh Mayadah Shehadeh Ghadeer A. R. Y. Suaifan Nida Karameh Diana Abdel Rahman Yasser Kandil In Vitro and In Vivo Antidiabetic Activity, Phenolic Content and Microscopical Characterization of <i>Terfezia claveryi</i> Molecules diabetes truffle <i>Terfezia claveryi</i> total phenols α-glucosidase inhibition antihyperglycemic |
| title | In Vitro and In Vivo Antidiabetic Activity, Phenolic Content and Microscopical Characterization of <i>Terfezia claveryi</i> |
| title_full | In Vitro and In Vivo Antidiabetic Activity, Phenolic Content and Microscopical Characterization of <i>Terfezia claveryi</i> |
| title_fullStr | In Vitro and In Vivo Antidiabetic Activity, Phenolic Content and Microscopical Characterization of <i>Terfezia claveryi</i> |
| title_full_unstemmed | In Vitro and In Vivo Antidiabetic Activity, Phenolic Content and Microscopical Characterization of <i>Terfezia claveryi</i> |
| title_short | In Vitro and In Vivo Antidiabetic Activity, Phenolic Content and Microscopical Characterization of <i>Terfezia claveryi</i> |
| title_sort | in vitro and in vivo antidiabetic activity phenolic content and microscopical characterization of i terfezia claveryi i |
| topic | diabetes truffle <i>Terfezia claveryi</i> total phenols α-glucosidase inhibition antihyperglycemic |
| url | https://www.mdpi.com/1420-3049/27/15/4843 |
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