T-RHEX-RNAseq – a tagmentation-based, rRNA blocked, random hexamer primed RNAseq method for generating stranded RNAseq libraries directly from very low numbers of lysed cells

T-RHEX-RNAseq – a tagmentation-based, rRNA blocked, random hexamer primed RNAseq method for generating stranded RNAseq libraries directly from very low numbers of lysed cells

Abstract Background RNA sequencing has become the mainstay for studies of gene expression. Still, analysis of rare cells with random hexamer priming – to allow analysis of a broader range of transcripts – remains challenging. Results We here describe a tagmentation-based, rRNA blocked, random hexame...

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Bibliographic Details
Main Authors: Charlotte Gustafsson, Julia Hauenstein, Nicolai Frengen, Aleksandra Krstic, Sidinh Luc, Robert Månsson
Format: Article
Language:English
Published: BMC 2023-04-01
Series:BMC Genomics
Subjects:
Stranded RNAseq
Tagmentation
Random hexamer priming
RNA purification free
Expression profiling
Online Access:https://doi.org/10.1186/s12864-023-09279-4

Internet

https://doi.org/10.1186/s12864-023-09279-4

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