The synergistic and enhancive effects of IL-6 and M-CSF to expand and differentiate functional dendritic cells from human monocytes under serum-free condition
Abstract Background Dendritic cells (DCs) are differentiated from monocytes, and have a strong ability to perform phagocytosis, present antigens and activate T cell immune response. Therefore, DCs are one of the key factors in fighting cancer in immunotherapy, and it is an important issue to develop...
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| Format: | Article |
| Language: | English |
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BMC
2023-01-01
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| Series: | Journal of Biological Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13036-023-00325-z |
| _version_ | 1828051714816606208 |
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| author | Chao-Ling Yao Tsung-Yu Tseng |
| author_facet | Chao-Ling Yao Tsung-Yu Tseng |
| author_sort | Chao-Ling Yao |
| collection | DOAJ |
| description | Abstract Background Dendritic cells (DCs) are differentiated from monocytes, and have a strong ability to perform phagocytosis, present antigens and activate T cell immune response. Therefore, DCs are one of the key factors in fighting cancer in immunotherapy, and it is an important issue to develop a serum-free system for DC differentiation and expansion in vitro for clinical application. Results In this study, IL-6 and M-CSF were determined and a concentration combination of cytokines was optimized to develop an optimal DC serum-free differentiation medium (SF-DC Optimal) that can effectively differentiate CD14+ monocytes into CD40+CD209+ DCs. After differentiation, the morphology, growth kinetics, surface antigen expression, phagocytosis ability, cytokine secretion, mixed lymphocyte reaction and stimulation for maturation of the differentiated DCs were checked and confirmed. Importantly, this research is the first report finding that the addition an extra low concentration of IL-6 and M-CSF exhibited a synergistic effect with GM-CSF and IL-4 to generate higher numbers and more fully functional DCs than the addition of GM-CSF and IL-4 only under serum-free condition. Conclusion A large number of functional DCs can be generated by using SF-DC Optimal medium and provide an alternative source of DCs for related basic research and clinical applications. |
| first_indexed | 2024-04-10T19:41:51Z |
| format | Article |
| id | doaj.art-478b67fc6de44f508186a25366223935 |
| institution | Directory Open Access Journal |
| issn | 1754-1611 |
| language | English |
| last_indexed | 2024-04-10T19:41:51Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biological Engineering |
| spelling | doaj.art-478b67fc6de44f508186a253662239352023-01-29T12:14:26ZengBMCJournal of Biological Engineering1754-16112023-01-0117111610.1186/s13036-023-00325-zThe synergistic and enhancive effects of IL-6 and M-CSF to expand and differentiate functional dendritic cells from human monocytes under serum-free conditionChao-Ling Yao0Tsung-Yu Tseng1Department of Chemical Engineering, National Cheng Kung UniversityDepartment of Chemical Engineering, National Cheng Kung UniversityAbstract Background Dendritic cells (DCs) are differentiated from monocytes, and have a strong ability to perform phagocytosis, present antigens and activate T cell immune response. Therefore, DCs are one of the key factors in fighting cancer in immunotherapy, and it is an important issue to develop a serum-free system for DC differentiation and expansion in vitro for clinical application. Results In this study, IL-6 and M-CSF were determined and a concentration combination of cytokines was optimized to develop an optimal DC serum-free differentiation medium (SF-DC Optimal) that can effectively differentiate CD14+ monocytes into CD40+CD209+ DCs. After differentiation, the morphology, growth kinetics, surface antigen expression, phagocytosis ability, cytokine secretion, mixed lymphocyte reaction and stimulation for maturation of the differentiated DCs were checked and confirmed. Importantly, this research is the first report finding that the addition an extra low concentration of IL-6 and M-CSF exhibited a synergistic effect with GM-CSF and IL-4 to generate higher numbers and more fully functional DCs than the addition of GM-CSF and IL-4 only under serum-free condition. Conclusion A large number of functional DCs can be generated by using SF-DC Optimal medium and provide an alternative source of DCs for related basic research and clinical applications.https://doi.org/10.1186/s13036-023-00325-zDendritic cellSerum-freeIL-6M-CSFImmunotherapy |
| spellingShingle | Chao-Ling Yao Tsung-Yu Tseng The synergistic and enhancive effects of IL-6 and M-CSF to expand and differentiate functional dendritic cells from human monocytes under serum-free condition Journal of Biological Engineering Dendritic cell Serum-free IL-6 M-CSF Immunotherapy |
| title | The synergistic and enhancive effects of IL-6 and M-CSF to expand and differentiate functional dendritic cells from human monocytes under serum-free condition |
| title_full | The synergistic and enhancive effects of IL-6 and M-CSF to expand and differentiate functional dendritic cells from human monocytes under serum-free condition |
| title_fullStr | The synergistic and enhancive effects of IL-6 and M-CSF to expand and differentiate functional dendritic cells from human monocytes under serum-free condition |
| title_full_unstemmed | The synergistic and enhancive effects of IL-6 and M-CSF to expand and differentiate functional dendritic cells from human monocytes under serum-free condition |
| title_short | The synergistic and enhancive effects of IL-6 and M-CSF to expand and differentiate functional dendritic cells from human monocytes under serum-free condition |
| title_sort | synergistic and enhancive effects of il 6 and m csf to expand and differentiate functional dendritic cells from human monocytes under serum free condition |
| topic | Dendritic cell Serum-free IL-6 M-CSF Immunotherapy |
| url | https://doi.org/10.1186/s13036-023-00325-z |
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