Eicosanoid Isolation from Mouse Intestinal Tissue for ELISA
Activation of inflammasomes in peritoneal macrophages and intestinal epithelial cells (IEC) leads to the release of eicosanoids. To assess the amount of eicosanoids released by IEC, lipids need to be isolated from whole tissue previous to analysis by lipid mass spectrometry or ELISA. This protocol d...
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| Format: | Article |
| Language: | English |
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Bio-protocol LLC
2018-11-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=3066&type=0 |