Solubilization, purification, and ligand binding characterization of G protein-coupled receptor SMO in native membrane bilayer using styrene maleic acid copolymer

Solubilization, purification, and ligand binding characterization of G protein-coupled receptor SMO in native membrane bilayer using styrene maleic acid copolymer

Smoothened (SMO) protein is a member of the G protein-coupled receptor (GPCR) family that is involved in the Hedgehog (Hh) signaling pathway. It is a putative target for treating various cancers, including medulloblastoma and basal cell carcinoma (BCC). Characterizing membrane proteins such as SMO i...

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Bibliographic Details
Main Authors: Lina Zhu, Hongxin Zhao, Yizhuo Wang, Chuandi Yu, Juanjuan Liu, Ling Li, Zehua Li, Jin Zhang, Han Dai, Junfeng Wang, Lei Zhu
Format: Article
Language:English
Published: PeerJ Inc. 2022-05-01
Series:PeerJ
Subjects:
GPCR
Dissociation constant
Detergents
Styrene maleic acid copolymer nanodiscs
Smoothened receptors
NMR
Online Access:https://peerj.com/articles/13381.pdf

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https://peerj.com/articles/13381.pdf

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