Experimental evaluation of super-resolution imaging and magnification choice in single-particle cryo-EM

The resolution of cryo-EM reconstructions is fundamentally limited by the Nyquist frequency, which is half the sampling frequency of the detector and depends upon the magnification used. In principle, super-resolution imaging should enable reconstructions to surpass the physical Nyquist limit by inc...

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Bibliographic Details
Main Authors: J. Ryan Feathers, Katherine A. Spoth, J. Christopher Fromme
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:Journal of Structural Biology: X
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2590152421000040