A Multicolor Fluorescence in situ Hybridization Approach Using an Extended Set of Fluorophores to Visualize Microorganisms

A Multicolor Fluorescence in situ Hybridization Approach Using an Extended Set of Fluorophores to Visualize Microorganisms

Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes is a key method for the detection of (uncultured) microorganisms in environmental and medical samples. A major limitation of standard FISH protocols, however, is the small number of phylogenetically distinct target o...

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Bibliographic Details
Main Authors: Michael Lukumbuzya, Markus Schmid, Petra Pjevac, Holger Daims
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-06-01
Series:Frontiers in Microbiology
Subjects:
fluorescence in situ hybridization
confocal laser scanning microscopy
white light laser
fluorophores
microorganisms
three-dimensional structure
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.01383/full

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https://www.frontiersin.org/article/10.3389/fmicb.2019.01383/full

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