In Vitro Evaluation of the Influence of Extraction Solvents on Antioxidant and Anti-Inflammatory Potentials of Dried Leaf of Bryophyllum pinnatum Lam.
This study investigated the effect of different extraction solvents on the phenolic contents (TPC), antioxidant activities, and anti-inflammatory potentials of dried-leaf extracts of Bryophyllum pinnatum Lam. The analyses were performed spectrophotometrically, using a Folin-Ciocalteau reagent for th...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2022-06-01
|
| Series: | Contemporary Agriculture |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/contagri-2023-0010 |
| _version_ | 1797815362875555840 |
|---|---|
| author | Oso Babatunde J. Abey Nosarieme O. Oyeleke Oyedotun M. Olowookere Boyede |
| author_facet | Oso Babatunde J. Abey Nosarieme O. Oyeleke Oyedotun M. Olowookere Boyede |
| author_sort | Oso Babatunde J. |
| collection | DOAJ |
| description | This study investigated the effect of different extraction solvents on the phenolic contents (TPC), antioxidant activities, and anti-inflammatory potentials of dried-leaf extracts of Bryophyllum pinnatum Lam. The analyses were performed spectrophotometrically, using a Folin-Ciocalteau reagent for the estimation of the total phenolic contents. The ferric reducing power (FRP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays were carried out to assess the antioxidant activities of aqueous, ethanolic, ethyl acetate, and n-hexane extracts of the plant, whereas the stabilization of heat-treated albumin and inhibition of protease action were performed to evaluate the anti-inflammatory potentials of the extracts. The results obtained indicate that the solvent nature significantly influenced the amount of TPC, antioxidant and anti-inflammatory potentials of the extracts. The aqueous extract had the highest TPC. The FRP was equally high in the aqueous and ethanol extracts. The ethanolic extract had the highest radical scavenging activity and anti-inflammatory potentials. The results show that water and ethanol could be suitable solvents for the extraction of bioactive compounds from the dried leaf of B. pinnatum vis-à-vis antioxidant and anti-inflammatory potentials. |
| first_indexed | 2024-03-13T08:22:30Z |
| format | Article |
| id | doaj.art-479bf8b9fd2e4346be3b20d08db1c27c |
| institution | Directory Open Access Journal |
| issn | 2466-4774 |
| language | English |
| last_indexed | 2024-03-13T08:22:30Z |
| publishDate | 2022-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Contemporary Agriculture |
| spelling | doaj.art-479bf8b9fd2e4346be3b20d08db1c27c2023-05-31T07:00:01ZengSciendoContemporary Agriculture2466-47742022-06-01721-2758110.2478/contagri-2023-0010In Vitro Evaluation of the Influence of Extraction Solvents on Antioxidant and Anti-Inflammatory Potentials of Dried Leaf of Bryophyllum pinnatum Lam.Oso Babatunde J.0Abey Nosarieme O.1Oyeleke Oyedotun M.2Olowookere Boyede31Department of Biochemistry, McPherson University, Seriki-Sotayo, P.M.B 2094, Abeokuta, Ogun State, Nigeria2Department of Biochemistry, Kings University, Odeomu, Osun State, Nigeria2Department of Biochemistry, Kings University, Odeomu, Osun State, Nigeria2Department of Biochemistry, Kings University, Odeomu, Osun State, NigeriaThis study investigated the effect of different extraction solvents on the phenolic contents (TPC), antioxidant activities, and anti-inflammatory potentials of dried-leaf extracts of Bryophyllum pinnatum Lam. The analyses were performed spectrophotometrically, using a Folin-Ciocalteau reagent for the estimation of the total phenolic contents. The ferric reducing power (FRP) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays were carried out to assess the antioxidant activities of aqueous, ethanolic, ethyl acetate, and n-hexane extracts of the plant, whereas the stabilization of heat-treated albumin and inhibition of protease action were performed to evaluate the anti-inflammatory potentials of the extracts. The results obtained indicate that the solvent nature significantly influenced the amount of TPC, antioxidant and anti-inflammatory potentials of the extracts. The aqueous extract had the highest TPC. The FRP was equally high in the aqueous and ethanol extracts. The ethanolic extract had the highest radical scavenging activity and anti-inflammatory potentials. The results show that water and ethanol could be suitable solvents for the extraction of bioactive compounds from the dried leaf of B. pinnatum vis-à-vis antioxidant and anti-inflammatory potentials.https://doi.org/10.2478/contagri-2023-0010bryophyllum pinnatum lam.total phenolicsantioxidant activityanti-inflammatorysolvent |
| spellingShingle | Oso Babatunde J. Abey Nosarieme O. Oyeleke Oyedotun M. Olowookere Boyede In Vitro Evaluation of the Influence of Extraction Solvents on Antioxidant and Anti-Inflammatory Potentials of Dried Leaf of Bryophyllum pinnatum Lam. Contemporary Agriculture bryophyllum pinnatum lam. total phenolics antioxidant activity anti-inflammatory solvent |
| title | In Vitro Evaluation of the Influence of Extraction Solvents on Antioxidant and Anti-Inflammatory Potentials of Dried Leaf of Bryophyllum pinnatum Lam. |
| title_full | In Vitro Evaluation of the Influence of Extraction Solvents on Antioxidant and Anti-Inflammatory Potentials of Dried Leaf of Bryophyllum pinnatum Lam. |
| title_fullStr | In Vitro Evaluation of the Influence of Extraction Solvents on Antioxidant and Anti-Inflammatory Potentials of Dried Leaf of Bryophyllum pinnatum Lam. |
| title_full_unstemmed | In Vitro Evaluation of the Influence of Extraction Solvents on Antioxidant and Anti-Inflammatory Potentials of Dried Leaf of Bryophyllum pinnatum Lam. |
| title_short | In Vitro Evaluation of the Influence of Extraction Solvents on Antioxidant and Anti-Inflammatory Potentials of Dried Leaf of Bryophyllum pinnatum Lam. |
| title_sort | in vitro evaluation of the influence of extraction solvents on antioxidant and anti inflammatory potentials of dried leaf of bryophyllum pinnatum lam |
| topic | bryophyllum pinnatum lam. total phenolics antioxidant activity anti-inflammatory solvent |
| url | https://doi.org/10.2478/contagri-2023-0010 |
| work_keys_str_mv | AT osobabatundej invitroevaluationoftheinfluenceofextractionsolventsonantioxidantandantiinflammatorypotentialsofdriedleafofbryophyllumpinnatumlam AT abeynosariemeo invitroevaluationoftheinfluenceofextractionsolventsonantioxidantandantiinflammatorypotentialsofdriedleafofbryophyllumpinnatumlam AT oyelekeoyedotunm invitroevaluationoftheinfluenceofextractionsolventsonantioxidantandantiinflammatorypotentialsofdriedleafofbryophyllumpinnatumlam AT olowookereboyede invitroevaluationoftheinfluenceofextractionsolventsonantioxidantandantiinflammatorypotentialsofdriedleafofbryophyllumpinnatumlam |