Prokaryotic expression of goldfish Tgf2 transposase with optimal codons and its enzyme activity
Tgf2 transposase (Tgf2-TPase), a hAT transposase from goldfish, plays an important role in fish transgenic applications. Previously, the production of the recombinant Tgf2-TPase protein required rigorous fermentation at low temperatures (22 °C) and early log phase induction (OD600 = 0.3–0.4) in Rose...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
KeAi Communications Co., Ltd.
2019-05-01
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Series: | Aquaculture and Fisheries |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2468550X18300479 |