Prokaryotic expression of goldfish Tgf2 transposase with optimal codons and its enzyme activity

Tgf2 transposase (Tgf2-TPase), a hAT transposase from goldfish, plays an important role in fish transgenic applications. Previously, the production of the recombinant Tgf2-TPase protein required rigorous fermentation at low temperatures (22 °C) and early log phase induction (OD600 = 0.3–0.4) in Rose...

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Bibliographic Details
Main Authors: Xi Zhao, Ruirui Si, Mingjuan He, Xiayun Jiang, Shuming Zou
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2019-05-01
Series:Aquaculture and Fisheries
Online Access:http://www.sciencedirect.com/science/article/pii/S2468550X18300479