Placental stem cells-derived exosomes stimulate cutaneous wound regeneration via engrailed-1 inhibition
Introduction: Skin wounds generally heal by scarring, a fibrotic process mediated by the Engrailed-1 (EN1) fibroblast lineage. Scar is detrimental to tissue structure and function, but perfect healing in clinical settings remains to be explored. Recent studies have shown that mesenchymal stem cell (...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1044773/full |
| _version_ | 1811178387935330304 |
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| author | Yan Zhang Liyan Shi Xiuying Li Yang Liu Guokun Zhang Yimin Wang |
| author_facet | Yan Zhang Liyan Shi Xiuying Li Yang Liu Guokun Zhang Yimin Wang |
| author_sort | Yan Zhang |
| collection | DOAJ |
| description | Introduction: Skin wounds generally heal by scarring, a fibrotic process mediated by the Engrailed-1 (EN1) fibroblast lineage. Scar is detrimental to tissue structure and function, but perfect healing in clinical settings remains to be explored. Recent studies have shown that mesenchymal stem cell (MSC) transplantation can reduce scarringMethods: Here, we investigated the effects of placental MSCs (pMSCs) and exosomes derived from pMSCs (pMSC-exos) on wound healing using a full-thickness rat model.Results: The results showed that placental MSCs significantly accelerated the wound healing rate. Moreover, placental MSCs improved the quality of wound healing, including regenerating cutaneous appendages (hair follicles and sebaceous glands), decreasing collagen I and increasing collagen III, and improving collagen pattern (basket-wave-like) in the healed skin. placental MSCs treatment also increased the regeneration of blood vessels. Importantly, all these listed effects of placental MSCs were comparable to those of exosomes derived from pMSCs, but significantly stronger than those of adipose MSC-derived exosomes (aMSC-exos). Further studies showed that the effects of placental MSCs and exosomes derived from pMSCs on wound regeneration may be mainly achieved via the down-regulation of the Yes-associated protein signaling pathway, thus inhibiting the activation of EN1. Discussion: In summary, placental MSCs could effectively stimulate wound regeneration, and their effect could be achieved through their exosomes. This suggests that exosomes derived from pMSCs treatment could be used as a novel cell-free approach to induce wound regeneration in clinical settings. |
| first_indexed | 2024-04-11T06:18:21Z |
| format | Article |
| id | doaj.art-47afadba86124dacb729db6d189c04e1 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-11T06:18:21Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-47afadba86124dacb729db6d189c04e12022-12-22T04:40:59ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-12-011010.3389/fbioe.2022.10447731044773Placental stem cells-derived exosomes stimulate cutaneous wound regeneration via engrailed-1 inhibitionYan Zhang0Liyan Shi1Xiuying Li2Yang Liu3Guokun Zhang4Yimin Wang5China-Japan Union Hospital of Jilin University, Changchun, ChinaChina-Japan Union Hospital of Jilin University, Changchun, ChinaChina-Japan Union Hospital of Jilin University, Changchun, ChinaJilin Province People’s Hospital, Changchun, ChinaInstitute of Antler Science and Product Technology, Changchun Sci-Tech University, Changchun, ChinaChina-Japan Union Hospital of Jilin University, Changchun, ChinaIntroduction: Skin wounds generally heal by scarring, a fibrotic process mediated by the Engrailed-1 (EN1) fibroblast lineage. Scar is detrimental to tissue structure and function, but perfect healing in clinical settings remains to be explored. Recent studies have shown that mesenchymal stem cell (MSC) transplantation can reduce scarringMethods: Here, we investigated the effects of placental MSCs (pMSCs) and exosomes derived from pMSCs (pMSC-exos) on wound healing using a full-thickness rat model.Results: The results showed that placental MSCs significantly accelerated the wound healing rate. Moreover, placental MSCs improved the quality of wound healing, including regenerating cutaneous appendages (hair follicles and sebaceous glands), decreasing collagen I and increasing collagen III, and improving collagen pattern (basket-wave-like) in the healed skin. placental MSCs treatment also increased the regeneration of blood vessels. Importantly, all these listed effects of placental MSCs were comparable to those of exosomes derived from pMSCs, but significantly stronger than those of adipose MSC-derived exosomes (aMSC-exos). Further studies showed that the effects of placental MSCs and exosomes derived from pMSCs on wound regeneration may be mainly achieved via the down-regulation of the Yes-associated protein signaling pathway, thus inhibiting the activation of EN1. Discussion: In summary, placental MSCs could effectively stimulate wound regeneration, and their effect could be achieved through their exosomes. This suggests that exosomes derived from pMSCs treatment could be used as a novel cell-free approach to induce wound regeneration in clinical settings.https://www.frontiersin.org/articles/10.3389/fbioe.2022.1044773/fullplacental MSCsexosomeswound regenerationSCARengrailed-1 |
| spellingShingle | Yan Zhang Liyan Shi Xiuying Li Yang Liu Guokun Zhang Yimin Wang Placental stem cells-derived exosomes stimulate cutaneous wound regeneration via engrailed-1 inhibition Frontiers in Bioengineering and Biotechnology placental MSCs exosomes wound regeneration SCAR engrailed-1 |
| title | Placental stem cells-derived exosomes stimulate cutaneous wound regeneration via engrailed-1 inhibition |
| title_full | Placental stem cells-derived exosomes stimulate cutaneous wound regeneration via engrailed-1 inhibition |
| title_fullStr | Placental stem cells-derived exosomes stimulate cutaneous wound regeneration via engrailed-1 inhibition |
| title_full_unstemmed | Placental stem cells-derived exosomes stimulate cutaneous wound regeneration via engrailed-1 inhibition |
| title_short | Placental stem cells-derived exosomes stimulate cutaneous wound regeneration via engrailed-1 inhibition |
| title_sort | placental stem cells derived exosomes stimulate cutaneous wound regeneration via engrailed 1 inhibition |
| topic | placental MSCs exosomes wound regeneration SCAR engrailed-1 |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1044773/full |
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