Visualizing orthogonal RNAs simultaneously in live mammalian cells by fluorescence lifetime imaging microscopy (FLIM)
No multi-color RNA fluorescent tags are currently available for use in live cells. Here, the authors show that fluorescence lifetime imaging microscopy is advantageous for multiplexed RNA visualization while achieving robust cellular contrast.
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2023-02-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-023-36531-y |