Gene Editing in Human Lymphoid Cells: Role for Donor DNA, Type of Genomic Nuclease and Cell Selection Method

Gene Editing in Human Lymphoid Cells: Role for Donor DNA, Type of Genomic Nuclease and Cell Selection Method

Programmable endonucleases introduce DNA breaks at specific sites, which are repaired by non-homologous end joining (NHEJ) or homology recombination (HDR). Genome editing in human lymphoid cells is challenging as these difficult-to-transfect cells may also inefficiently repair DNA by HDR. Here, we e...

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Bibliographic Details
Main Authors: Anastasia Zotova, Elena Lopatukhina, Alexander Filatov, Musa Khaitov, Dmitriy Mazurov
Format: Article
Language:English
Published: MDPI AG 2017-11-01
Series:Viruses
Subjects:
CRISPR-Cas9
ZFN
gene editing
HIV-1
T lymphocytes
knockout
knockin
cell sorting
cell functionality
Online Access:https://www.mdpi.com/1999-4915/9/11/325

Internet

https://www.mdpi.com/1999-4915/9/11/325

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