Rapid Determination of RNA Modifications in Consensus Motifs by Nuclease Protection with Ion-Tagged Oligonucleotide Probes and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

Rapid Determination of RNA Modifications in Consensus Motifs by Nuclease Protection with Ion-Tagged Oligonucleotide Probes and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

The reversible and substoichiometric modification of RNA has recently emerged as an additional layer of translational regulation in normal biological function and disease. Modifications are often enzymatically deposited in and removed from short (~5 nt) consensus motif sequences to carefully control...

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Bibliographic Details
Main Authors: Madeline E. Melzer, Jonathan V. Sweedler, Kevin D. Clark
Format: Article
Language:English
Published: MDPI AG 2022-06-01
Series:Genes
Subjects:
RNA modifications
matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS)
m<sup>6</sup>A consensus motif
ion-tagged oligonucleotides (ITOs)
RNA modification stoichiometry
nuclease protection
Online Access:https://www.mdpi.com/2073-4425/13/6/1008

Internet

https://www.mdpi.com/2073-4425/13/6/1008

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