Generation of orthotopic patient-derived xenograft models for pancreatic cancer using tumor slices
Summary: Orthotopic patient-derived xenograft models recapitulate the genomic complexity of the original tumor and some aspects of local microenvironment, useful for rapid development and validation of personalized treatment strategies. Here, we precisely describe a protocol for generating tumor sli...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722007791 |
| _version_ | 1811199603041632256 |
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| author | Alvaro Curiel-Garcia Amanda R. Decker-Farrell Stephen A. Sastra Kenneth P. Olive |
| author_facet | Alvaro Curiel-Garcia Amanda R. Decker-Farrell Stephen A. Sastra Kenneth P. Olive |
| author_sort | Alvaro Curiel-Garcia |
| collection | DOAJ |
| description | Summary: Orthotopic patient-derived xenograft models recapitulate the genomic complexity of the original tumor and some aspects of local microenvironment, useful for rapid development and validation of personalized treatment strategies. Here, we precisely describe a protocol for generating tumor slices from human or murine-derived pancreatic cancer. They are then implanted directly into the murine pancreas, monitored using ultrasound, with a 90% success rate. This assay creates a clinically relevant in vivo model facilitating personalized treatment development. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-12T01:51:28Z |
| format | Article |
| id | doaj.art-4820c73bad6d497cb562dbb9bc35ca66 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-12T01:51:28Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-4820c73bad6d497cb562dbb9bc35ca662022-12-22T03:52:56ZengElsevierSTAR Protocols2666-16672022-12-0134101899Generation of orthotopic patient-derived xenograft models for pancreatic cancer using tumor slicesAlvaro Curiel-Garcia0Amanda R. Decker-Farrell1Stephen A. Sastra2Kenneth P. Olive3Division of Digestive and Liver Diseases, Department of Medicine, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY 10032, USA; Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 1032, USA; Corresponding authorDivision of Digestive and Liver Diseases, Department of Medicine, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY 10032, USA; Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 1032, USADivision of Digestive and Liver Diseases, Department of Medicine, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY 10032, USA; Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 1032, USADivision of Digestive and Liver Diseases, Department of Medicine, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY 10032, USA; Herbert Irving Comprehensive Cancer Center, Columbia University Irving Medical Center, New York, NY 1032, USA; Corresponding authorSummary: Orthotopic patient-derived xenograft models recapitulate the genomic complexity of the original tumor and some aspects of local microenvironment, useful for rapid development and validation of personalized treatment strategies. Here, we precisely describe a protocol for generating tumor slices from human or murine-derived pancreatic cancer. They are then implanted directly into the murine pancreas, monitored using ultrasound, with a 90% success rate. This assay creates a clinically relevant in vivo model facilitating personalized treatment development. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722007791CancerModel Organisms |
| spellingShingle | Alvaro Curiel-Garcia Amanda R. Decker-Farrell Stephen A. Sastra Kenneth P. Olive Generation of orthotopic patient-derived xenograft models for pancreatic cancer using tumor slices STAR Protocols Cancer Model Organisms |
| title | Generation of orthotopic patient-derived xenograft models for pancreatic cancer using tumor slices |
| title_full | Generation of orthotopic patient-derived xenograft models for pancreatic cancer using tumor slices |
| title_fullStr | Generation of orthotopic patient-derived xenograft models for pancreatic cancer using tumor slices |
| title_full_unstemmed | Generation of orthotopic patient-derived xenograft models for pancreatic cancer using tumor slices |
| title_short | Generation of orthotopic patient-derived xenograft models for pancreatic cancer using tumor slices |
| title_sort | generation of orthotopic patient derived xenograft models for pancreatic cancer using tumor slices |
| topic | Cancer Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722007791 |
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