Protocol for the microinjection of free fatty acids and triacylglycerol in zebrafish embryos

Summary: During development, the zebrafish embryo relies on its yolk sac as a nutrient source. Here, we present a protocol for modifying the free fatty acid (FFA) and triacylglycerol (TAG) content of the zebrafish yolk sac by microinjection. We describe steps for needle and injection mold preparatio...

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Bibliographic Details
Main Authors: Bridget Konadu, Carol K. Cox, Yann Gibert
Format: Article
Language:English
Published: Elsevier 2024-06-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672400251X