Active Immunization with Extracellular Vesicles Derived from Staphylococcus aureus Effectively Protects against Staphylococcal Lung Infections, Mainly via Th1 Cell-Mediated Immunity.
Staphylococcus aureus is an important pathogenic bacterium that causes various infectious diseases. Extracellular vesicles (EVs) released from S. aureus contain bacterial proteins, nucleic acids, and lipids. These EVs can induce immune responses leading to similar symptoms as during staphylococcal i...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4558092?pdf=render |
| _version_ | 1818278338291040256 |
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| author | Seng Jin Choi Min-Hye Kim Jinseong Jeon Oh Youn Kim Youngwoo Choi Jihye Seo Sung-Wook Hong Won-Hee Lee Seong Gyu Jeon Yong Song Gho Young-Koo Jee Yoon-Keun Kim |
| author_facet | Seng Jin Choi Min-Hye Kim Jinseong Jeon Oh Youn Kim Youngwoo Choi Jihye Seo Sung-Wook Hong Won-Hee Lee Seong Gyu Jeon Yong Song Gho Young-Koo Jee Yoon-Keun Kim |
| author_sort | Seng Jin Choi |
| collection | DOAJ |
| description | Staphylococcus aureus is an important pathogenic bacterium that causes various infectious diseases. Extracellular vesicles (EVs) released from S. aureus contain bacterial proteins, nucleic acids, and lipids. These EVs can induce immune responses leading to similar symptoms as during staphylococcal infection condition and have the potential as vaccination agent. Here, we show that active immunization (vaccination) with S. aureus-derived EVs induce adaptive immunity of antibody and T cell responses. In addition, these EVs have the vaccine adjuvant ability to induce protective immunity such as the up-regulation of co-stimulatory molecules and the expression of T cell polarizing cytokines in antigen-presenting cells. Moreover, vaccination with S. aureus EVs conferred protection against lethality induced by airway challenge with lethal dose of S. aureus and also pneumonia induced by the administration of sub-lethal dose of S. aureus. These protective effects were also found in mice that were adoptively transferred with splenic T cells isolated from S. aureus EV-immunized mice, but not in serum transferred mice. Furthermore, this protective effect of S. aureus EVs was significantly reduced by the absence of interferon-gamma, but not by the absence of interleukin-17. Together, the study herein suggests that S. aureus EVs are a novel vaccine candidate against S. aureus infections, mainly via Th1 cellular response. |
| first_indexed | 2024-12-12T23:15:51Z |
| format | Article |
| id | doaj.art-482ca176c41b4710b7f20840c93415ca |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T23:15:51Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-482ca176c41b4710b7f20840c93415ca2022-12-22T00:08:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01109e013602110.1371/journal.pone.0136021Active Immunization with Extracellular Vesicles Derived from Staphylococcus aureus Effectively Protects against Staphylococcal Lung Infections, Mainly via Th1 Cell-Mediated Immunity.Seng Jin ChoiMin-Hye KimJinseong JeonOh Youn KimYoungwoo ChoiJihye SeoSung-Wook HongWon-Hee LeeSeong Gyu JeonYong Song GhoYoung-Koo JeeYoon-Keun KimStaphylococcus aureus is an important pathogenic bacterium that causes various infectious diseases. Extracellular vesicles (EVs) released from S. aureus contain bacterial proteins, nucleic acids, and lipids. These EVs can induce immune responses leading to similar symptoms as during staphylococcal infection condition and have the potential as vaccination agent. Here, we show that active immunization (vaccination) with S. aureus-derived EVs induce adaptive immunity of antibody and T cell responses. In addition, these EVs have the vaccine adjuvant ability to induce protective immunity such as the up-regulation of co-stimulatory molecules and the expression of T cell polarizing cytokines in antigen-presenting cells. Moreover, vaccination with S. aureus EVs conferred protection against lethality induced by airway challenge with lethal dose of S. aureus and also pneumonia induced by the administration of sub-lethal dose of S. aureus. These protective effects were also found in mice that were adoptively transferred with splenic T cells isolated from S. aureus EV-immunized mice, but not in serum transferred mice. Furthermore, this protective effect of S. aureus EVs was significantly reduced by the absence of interferon-gamma, but not by the absence of interleukin-17. Together, the study herein suggests that S. aureus EVs are a novel vaccine candidate against S. aureus infections, mainly via Th1 cellular response.http://europepmc.org/articles/PMC4558092?pdf=render |
| spellingShingle | Seng Jin Choi Min-Hye Kim Jinseong Jeon Oh Youn Kim Youngwoo Choi Jihye Seo Sung-Wook Hong Won-Hee Lee Seong Gyu Jeon Yong Song Gho Young-Koo Jee Yoon-Keun Kim Active Immunization with Extracellular Vesicles Derived from Staphylococcus aureus Effectively Protects against Staphylococcal Lung Infections, Mainly via Th1 Cell-Mediated Immunity. PLoS ONE |
| title | Active Immunization with Extracellular Vesicles Derived from Staphylococcus aureus Effectively Protects against Staphylococcal Lung Infections, Mainly via Th1 Cell-Mediated Immunity. |
| title_full | Active Immunization with Extracellular Vesicles Derived from Staphylococcus aureus Effectively Protects against Staphylococcal Lung Infections, Mainly via Th1 Cell-Mediated Immunity. |
| title_fullStr | Active Immunization with Extracellular Vesicles Derived from Staphylococcus aureus Effectively Protects against Staphylococcal Lung Infections, Mainly via Th1 Cell-Mediated Immunity. |
| title_full_unstemmed | Active Immunization with Extracellular Vesicles Derived from Staphylococcus aureus Effectively Protects against Staphylococcal Lung Infections, Mainly via Th1 Cell-Mediated Immunity. |
| title_short | Active Immunization with Extracellular Vesicles Derived from Staphylococcus aureus Effectively Protects against Staphylococcal Lung Infections, Mainly via Th1 Cell-Mediated Immunity. |
| title_sort | active immunization with extracellular vesicles derived from staphylococcus aureus effectively protects against staphylococcal lung infections mainly via th1 cell mediated immunity |
| url | http://europepmc.org/articles/PMC4558092?pdf=render |
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