Harnessing accurate non-homologous end joining for efficient precise deletion in CRISPR/Cas9-mediated genome editing

Harnessing accurate non-homologous end joining for efficient precise deletion in CRISPR/Cas9-mediated genome editing

Abstract Background Many applications of CRISPR/Cas9-mediated genome editing require Cas9-induced non-homologous end joining (NHEJ), which was thought to be error prone. However, with directly ligatable ends, Cas9-induced DNA double strand breaks may be repaired preferentially by accurate NHEJ. Resu...

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Bibliographic Details
Main Authors: Tao Guo, Yi-Li Feng, Jing-Jing Xiao, Qian Liu, Xiu-Na Sun, Ji-Feng Xiang, Na Kong, Si-Cheng Liu, Guo-Qiao Chen, Yue Wang, Meng-Meng Dong, Zhen Cai, Hui Lin, Xiu-Jun Cai, An-Yong Xie
Format: Article
Language:English
Published: BMC 2018-10-01
Series:Genome Biology
Subjects:
Paired gRNAs
CRISPR/Cas9
Accurate NHEJ
Templated insertions
Precise deletion
Knockout
Online Access:http://link.springer.com/article/10.1186/s13059-018-1518-x

Internet

http://link.springer.com/article/10.1186/s13059-018-1518-x

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